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- Table of Contents
BRAF antibody (A00075) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human, Mouse.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of B-raf in Human Small Intestine Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Small Intestine Tissue labeling B-raf with A00075 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Small Intestine Tissue labeling B-raf with A00075 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Anti-B-raf Antibody
BRAF antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 0.5-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-B-raf Antibody
View all BRAF Antibodies
View all BRAF Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
B-raf antibody can be stored at 4°C, stable for up to one year.
Host
Rabbit
Contents
B-raf Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-B-raf antibody was raised against a peptide corresponding to 19 amino acids near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf.
Cross-Reactivity
This antibody will not cross-react with C-raf.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
84.4 kDa
Background of BRAF
B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
Cite This Product
Anti-B-raf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00075)
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Product Promise
We promise Anti-B-raf Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00075
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026