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Product Info Summary
| SKU: | A00075 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-B-raf Antibody
SKU/Catalog Number
A00075
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-B-raf Antibody (Catalog # A00075). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
B-raf antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-B-raf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00075)
Host
Rabbit
Contents
B-raf Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-B-raf antibody was raised against a peptide corresponding to 19 amino acids near the center of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf.
Cross-reactivity
This antibody will not cross-react with C-raf.
Reactive Species
A00075 is reactive to BRAF in Human, Mouse
Observed Molecular Weight
68 kDa
Calculated molecular weight
84.4 kDa
Background of BRAF
B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00075 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 0.5-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation in Human Brain Tissue
Loading: 15 μg of lysates per lane.
Antibodies: B-raf A00075, (A:1 μg/mL; B:2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane.
Antibodies: B-raf 5777, (0.5 μg/mL), B-raf A00075, (0.5 μg/mL) and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.
Secondary: Goat anti-chicken (for 5777) and goat anti-rabbit (for A00075) IgG HRP conjugate at 1:10000 dilution.
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Immunohistochemistry Validation of B-raf in Human Small Intestine Tissue
Immunohistochemical analysis of paraffin-embedded Human Small Intestine Tissue using anti-B-raf antibody (A00075) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunofluorescence Validation of B-raf in Human Small Intestine Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Small Intestine Tissue labeling B-raf with A00075 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Specific Publications For Anti-B-raf Antibody (A00075)
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