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- Table of Contents
Designing a Western blot for BRAF? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the BRAF Western Blot Guide2 Citations
Facts about Serine/threonine-protein kinase B-raf.
Phosphorylates MAP2K1, and thus contributes to the MAP kinase signal transduction pathway. .
| Human | |
|---|---|
| Gene Name: | BRAF |
| Uniprot: | P15056 |
| Entrez: | 673 |

| Belongs to: |
|---|
| protein kinase superfamily |

94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109
Mass (kDA):
84.437 kDA

| Human | |
|---|---|
| Location: | 7q34 |
| Sequence: | 7; NC_000007.14 (140713328..140924929, complement) |
Brain and testis.
Nucleus. Cytoplasm. Cell membrane. Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.






PMID: 1508179 by Stephens R.M., et al. 95-kilodalton B-Raf serine/threonine kinase: identification of the protein and its major autophosphorylation site.
PMID: 1630826 by Eychene A., et al. Chromosomal assignment of two human B-raf(Rmil) proto-oncogene loci: B-raf-1 encoding the p94Braf/Rmil and B-raf-2, a processed pseudogene.
*More publications can be found for each product on its corresponding product page