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Product Info Summary
| SKU: | A00456 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Beta-2-microglobulin B2M Antibody
SKU/Catalog Number
A00456
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Beta-2-microglobulin B2M Antibody (Catalog # A00456). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
B2M antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Beta-2-microglobulin B2M Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00456)
Host
Rabbit
Contents
B2M Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
B2M antibody was raised against a 15 amino acid peptide near the carboxy terminus of human B2M. The immunogen is located within the last 50 amino acids of B2M.
Cross-reactivity
B2M antibody is human reactive.
Reactive Species
A00456 is reactive to B2M in Human
Observed Molecular Weight
68 kDa
Calculated molecular weight
13.7 kDa
Background of B2M
Beta2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a ternary membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system (1,2). B2M is involved in the presentation of peptide antigens to the immune system and plays a critically important role in immune system function (3). It is expressed on nearly all nucleated cells and contains one Ig-like C1-type (immunoglobulin-like) domain (2,3). Mutations in the Beta 2-microglobulin gene can enhance the progression of malignant melanoma and osteoarthropathy (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00456 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
B2M antibody can be used for detection of B2M by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of B2M in SK-N-SH cell lysate with B2M antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of B2M in mouse brain tissue with B2M antibody 5 μg/mL.
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Immunofluorescence of B2M in mouse brain tissue with B2M antibody at 20 μg/mL.
Specific Publications For Anti-Beta-2-microglobulin B2M Antibody (A00456)
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