Product Info Summary
| SKU: | PB10048 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-BMP15 Antibody Picoband®
SKU/Catalog Number
PB10048
PB1103 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BMP15 Antibody Picoband® catalog # PB10048. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMP15 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10048)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human BMP15 recombinant protein (Position: Q268-R392). Human BMP15 shares 69.6% amino acid (aa) sequence identity with mouse BMP15.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10048 is reactive to BMP15 in Human
Observed Molecular Weight
56 kDa
Calculated molecular weight
45.1 kDa
Background of BMP15
Bone morphogenetic protein 15 is a protein that in humans is encoded by the BMP15 gene. It's mainly involved in folliculogenesis. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The transforming growth factor-beta superfamily includes large families of growth and differentiation factors. It is thought that this protein may be involved in oocyte maturation and follicular development as a homodimer or by forming heterodimers with a related protein, Gdf9. Defects in this gene are the cause of ovarian dysgenesis 2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10048 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: HELA whole cell, MCF-7 whole cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of BMP15 using anti-BMP15 antibody (PB10048).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: HELA whole cell lysates,
Lane 2: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMP15 antigen affinity purified polyclonal antibody (Catalog # PB10048) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMP15 at approximately 56 kDa. The expected band size for BMP15 is at 45 kDa.
Specific Publications For Anti-BMP15 Antibody Picoband® (PB10048)
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Customer Q&As
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6 Customer Q&As for Anti-BMP15 Antibody Picoband®
Question
Will anti-BMP15 antibody PB10048 work on primate WB with secondary oocyte?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Our lab technicians have not tested anti-BMP15 antibody PB10048 on primate. You can run a BLAST between primate and the immunogen sequence of anti-BMP15 antibody PB10048 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate secondary oocyte in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
I have attached the WB image, lot number and protocol we used for secondary oocyte using anti-BMP15 antibody PB10048. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-13
Question
We are currently using anti-BMP15 antibody PB10048 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The anti-BMP15 antibody (PB10048) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-05
Question
Does anti-BMP15 antibody PB10048 work for WB with secondary oocyte?
J. Huang
Verified customer
Asked: 2018-11-14
Answer
According to the expression profile of secondary oocyte, BMP15 is highly expressed in secondary oocyte. So, it is likely that anti-BMP15 antibody PB10048 will work for WB with secondary oocyte.
Boster Scientific Support
Answered: 2018-11-14
Question
I see that the anti-BMP15 antibody PB10048 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-06-01
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for secondary oocyte using anti-BMP15 antibody PB10048. Let me know if you need anything else.
O. Roberts
Verified customer
Asked: 2015-04-09
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-04-09


