Anti-SIAH Interacting Protein/CACYBP Antibody
Rabbit IgG polyclonal antibody for Calcyclin-binding protein(CACYBP) detection. Tested with WB, IHC-P, ICC in Human;Mouse;Rat.
Anti-SIAH Interacting Protein/CACYBP Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat|
|Applications:||IHC, ICC, WB|
|Product Name||Anti-SIAH Interacting Protein/CACYBP Antibody
See all CACYBP primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for SIAH INTERACTING PROTEIN/CACYBP detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. SIAH INTERACTING PROTEIN/CACYBP information: Molecular Weight: 26210 MW; Subcellular Localization: Nucleus . Cytoplasm . Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.|
|Cite This Product||Anti-SIAH Interacting Protein/CACYBP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1759)|
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|Anti-SIAH Interacting Protein/CACYBP Antibody may replace the following items: sc 104122|sc 166455|sc 98356|sc 166195|sc 104120|sc 166163.|
|Reactivity/Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
|Applications||IHC, ICC, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human CACYBP (160-175aa NTRWDYLTQVEKECKE), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CACYBP (Source: Uniprot.org, NCBI)
|NCBI Gene Id||27101|
|Species Of This Entry||Human|
|Protein Name||Calcyclin-binding protein|
|Alternative Names||CACYBP|CacyBP; calcyclin binding protein; GIG5; hCacyBP; MGC87971; RP1-102G20.6; S100A6-binding protein; S100A6BPgrowth-inhibiting gene 5 protein; Siah-interacting protein (SIP); Siah-interacting protein; SIPcalcyclin-binding protein|
|Post Tranlational Modifications||Phosphorylation; Ubiquitination; Dephosphorylation; Cleavage|
|Subcellular Localization||Nucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated.|
|Gene Location||1q25.1, on Chromasome 1, gene sequence: NC_000001.11 (174999435..175012027)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CACYBP (Source: Uniprot.org, NCBI)
|Protein Function||May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1).|
|Research Areas||Wnt Signaling Pathway|
|Related Diseases||Malignant Neoplasms; Neoplasms; Malignant Neoplasm Of Stomach; Stomach Neoplasms; Cell Transformation, Neoplastic; Neoplasm Metastasis; Malignant Paraganglionic Neoplasm; Carcinogenesis; Malignant Neoplasm Of Pancreas; Cell Invasion; Neuroblastoma; Carcinoma, Ehrlich Tumor; Carcinoma; Tissue Adhesions; Malignant Neoplasm Of Breast; Malignant Tumor Of Colon; Renal Cell Carcinoma; Mammary Neoplasms; Malignant Neoplasm Of Kidney|
|Related Pathways||Localization; Cell Cycle; Cell Proliferation; Proteolysis; Cell Growth; Cell Adhesion; Drug Resistance; Cell Migration; Virulence; Cell Cycle Arrest; Decidualization; Cell Motility; Pathogenesis; Myoblast Differentiation; Rna Interference; Protein Ubiquitination; Nuclear Import; Secretion|
|Background||CACYBP(Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short(SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.|
|Scientific References||PMID: 12006993 by Liu W.X., et al. Cloning and expression of human calcyclin binding protein (hCacyBP) gene.|
PMID: 11389839 by Matsuzawa S., et al. Siah-1, SIP, and Ebi collaborate in a novel pathway for beta-catenin degradation linked to p53 responses.
Our Boster Quality Guarantee for Anti-SIAH Interacting Protein/CACYBP Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti CACYBP (PA1759) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: SW620 Whole Cell Lysate at 40ug
Lane 7: 293T Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
ICC: A549 Cell
IHC(P): Rat Brain Tissue
IHC(P): Human Liver Cancer Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Note: sample size takes longer to process, average 5-7 days.
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