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Product Info Summary
| SKU: | PA1759 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, ICC, WB |
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Product info
Product Name
Anti-SIAH Interacting Protein/CACYBP Antibody Picoband®
SKU/Catalog Number
PA1759
BA3736 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SIAH Interacting Protein/CACYBP Antibody catalog # PA1759. Tested in IP, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SIAH Interacting Protein/CACYBP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1759)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CACYBP, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1759 is reactive to CACYBP in Human, Mouse, Rat
Observed Molecular Weight
27 kDa
Calculated molecular weight
26.2 kDa
Background of CACYBP
CACYBP (Calcyclin-binding protein), also called SIP, is a protein that in humans is encoded by the CACYBP gene. The full-length SIP cDNA encodes a predicted 228-amino acid protein. Sequence analysis of the shortest cDNA derived by 2-hybrid screening revealed an 8-amino acid difference in the deduced open reading frame followed by a stop codon, resulting in a predicted 80-amino acid protein, SIP-short (SIPS). The CACYBP gene is mapped on 1q25.1. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene. The C-terminal region of SIP that is homologous to SGT1 was able to complement defects in yeast strains containing SGT1 mutant alleles, demonstrating conservation of SGT1 and SIP protein function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1759 is guaranteed for Flow Cytometry, IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Positive Control
WB: human MCF-7 whole cell, human RT4 whole cell, human SW620 whole cell, human U251 whole cell, rat liver tissue, rat brain tissue, mouse liver tissue, mouse brain tissue
ICC/IF: Hela cell
FCM: HL-60 cell
IP: U251 cell
Validation Images & Assay Conditions
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Immunoprecipitating CACYBP in U251 whole cell lysate.
Western blot analysis of CACYBP using anti-CACYBP antibody (PA1759).
Lane 1: U251 whole cell lysates (30ug),
Lane 2: Rabbit control IgG instead of anti-CACYBP antibody in U251 whole cell lysate,
Lane 3: anti-CACYBP antibody (2μg) + U251 whole cell lysate (500μg).
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CACYBP antigen affinity purified polyclonal antibody (PA1759) at a dilution of 0.5 μg/mL and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1196-200). A specific band was detected for CACYBP at approximately 27 kDa. The expected band size for CACYBP is at 27 kDa.
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Western blot analysis of CACYBP using anti-CACYBP antibody (PA1759).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human RT4 whole cell lysates,
Lane 3: human SW620 whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACYBP antigen affinity purified polyclonal antibody (Catalog # PA1759) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CACYBP at approximately 27 kDa. The expected band size for CACYBP is at 27 kDa.
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IF analysis of CACYBP using anti-CACYBP antibody (PA1759).
CACYBP was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CACYBP Antibody (PA1759) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Flow Cytometry analysis of HL-60 cells using anti-CACYBP antibody (PA1759).
Overlay histogram showing HL-60 cells stained with PA1759 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CACYBP Antibody (PA1759, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Specific Publications For Anti-SIAH Interacting Protein/CACYBP Antibody Picoband® (PA1759)
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4 Customer Q&As for Anti-SIAH Interacting Protein/CACYBP Antibody Picoband®
Question
Is a blocking peptide available for product anti-SIAH Interacting Protein/CACYBP antibody (PA1759)?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
We do provide the blocking peptide for product anti-SIAH Interacting Protein/CACYBP antibody (PA1759). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-05-01
Question
Please see the WB image, lot number and protocol we used for brain skin using anti-SIAH Interacting Protein/CACYBP antibody PA1759. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-24
Question
Is this PA1759 anti-SIAH Interacting Protein/CACYBP antibody reactive to the isotypes of CACYBP?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
The immunogen of PA1759 anti-SIAH Interacting Protein/CACYBP antibody is A synthetic peptide corresponding to a sequence in the middle region of human CACYBP (160-175aa NTRWDYLTQVEKECKE), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-14
Question
Does anti-SIAH Interacting Protein/CACYBP antibody PA1759 work for WB with brain skin?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
According to the expression profile of brain skin, CACYBP is highly expressed in brain skin. So, it is likely that anti-SIAH Interacting Protein/CACYBP antibody PA1759 will work for WB with brain skin.
Boster Scientific Support
Answered: 2019-10-28
