This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CAMKK2 antibody (A03048) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IF analysis of CAMKK2 using anti-CAMKK2 antibody (A03048).
CAMKK2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-CAMKK2 Antibody (A03048) overnight at 4°C. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CAMKK2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/ml rabbit anti-CAMKK2 Antibody (A03048) overnight at 4°C. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Bundle Save
Anti-CAMKK2 Antibody Picoband®
CAMKK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
CAMKK2 antibody was positively detected by WB in:
human PC-3 whole cell, human Hela whole cell, human Jurkat whole cell, human THP-1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CAMKK2 antibody was positively detected by IHC in:
human colorectal adenocarcinoma tissue, human esophageal squamous carcinoma tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CAMKK2 antibody was positively detected by IF in:
human intestinal cancer tissue
CAMKK2 antibody was positively detected by ICC/IF in:
PC-3 cell, Hela cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
ELISA
FC
Human
Tested
Tested
Tested
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CAMKK2 Antibody Picoband®
View all CAMKK2 Antibodies
View all CAMKK2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CAMKK2 recombinant protein (Position: M1-G349).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
65 kDa
Calculated Molecular Weight
64.7 kDa
Background of CAMKK2
CAMKK2, Calcium/calmodulin-dependent protein kinase kinase 2, is an enzyme that in humans is encoded by the CAMKK2 gene. The product of this gene belongs to the serine/threonine-specific protein kinase family, and to the Ca++/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent(CaM) kinase cascade by phosphorylating the downstream kinases CaMK1 and CaMK4. CaMKK2 regulates production of the appetite stimulating hormone neuropeptide Y and functions as an AMPK kinase in the hypothalamus. It also has an important role in the development of hyperalgesia and tolerance to opioid analgesic drugs, through reduction in downstream signalling pathways and mu opioid receptor downregulation. Inhibition of CaMKK2 in mice reduces appetite and promotes weight loss.
Cite This Product
Anti-CAMKK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03048)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Rabbit
93%
High Homology
Rat
92%
High Homology
Cow
92%
High Homology
Sheep
92%
High Homology
Mouse
90%
High Homology
Dog
90%
High Homology
Zebrafish
70%
Moderate Homology
Drosophila
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CAMKK2 antibody
Immunohistochemistry (IHC) protocol for CAMKK2 antibody
Immunofluorescence (IF) protocol for CAMKK2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CAMKK2 antibody
Review
Have you used Anti-CAMKK2 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-CAMKK2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A03048
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026