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Product Info Summary
| SKU: | A05475-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Camp Antibody Picoband®
SKU/Catalog Number
A05475-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Camp Antibody Picoband® catalog # A05475-1. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Camp Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05475-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat Camp, which shares 83.3% amino acid (aa) sequence identity with mouse Camp.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05475-1 is reactive to Camp in Mouse, Rat
Observed Molecular Weight
20 kDa
Background of Camp
This gene encodes a member of an antimicrobial peptide family, characterized by a highly conserved N-terminal signal peptide containing a cathelin domain and a structurally variable cationic antimicrobial peptide, which is produced by extracellular proteolysis from the C-terminus. The protein plays an important role in innate immunity defense against viruses. In addition to its antibacterial, antifungal, and antiviral activities, the encoded protein functions in cell chemotaxis, immune mediator induction, and inflammatory response regulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05475-1 is guaranteed for WB Boster Guarantee
Recommend Dilution
| Application | Dilution | Species |
|---|---|---|
| Western Blot (WB) | 0.25-0.5 μg/ml | Mouse, Rat |
Tested application
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 µg per lane
Validation Images & Assay Conditions
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Western blot analysis of Camp using anti-Camp antibody (A05475-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Camp antigen affinity purified polyclonal antibody (Catalog # A05475-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Camp at approximately 20 kDa. The expected band size for Camp is at 20 kDa.
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Western blot analysis of Camp using anti-Camp antibody (A05475-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MADB106 cells under normal culture conditions,
Lane 2:MADB106 cells treated with agonist,
Lane 3:MADB106 cells treated with inhibitor.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Camp antigen affinity purified polyclonal antibody (Catalog # A05475-1) at 1:2000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with ChemiDoc MP system. A specific band was detected for Camp at approximately 17-20 kDa. The expected band size for Camp is at 20 kDa.
Specific Publications For Anti-Camp Antibody Picoband® (A05475-1)
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Customer Reviews
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1 Reviews For Anti-Camp Antibody Picoband®
In this WB experiment using Anti-CAMP antibody (Cat# A05475-1) on MADB106 rat mammary carcinoma cells, CAMP protein secretion was markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor.
Excellent
| SKU | A05475-1 |
|---|---|
| Application | Western blot |
| Sample | MADB106 rat mammary carcinoma cells |
| Sample Processing Description | MADB106 cells under normal culture conditions , MADB106 cells treated with agonist , MADB106 cells treated with inhibitor |
| Other Reagents | RIPA Lysis Buffer, Protease Inhibitor, Resolving Gel Solution ,Transfer Buffer ,Blocking Buffer |
| Primary Antibody | Camp Antibody Picoband® |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | 1:10,000, HRP-conjugated Goat Anti-Rabbit IgG |
| Secondary Incubation | 1h at 37℃ |
| Detection | Substrate: ECL substrate, Imaging system:ChemiDoc MP |
| Results Summary | CAMP, also known as cathelicidin antimicrobial peptide or LL-37, is an important antimicrobial peptide with dual functions: directly killing pathogens and modulating immune responses. It plays a complex role in maintaining the homeostasis of barrier tissues such as skin and in influencing tumor progression. In breast cancer, CAMP may promote tumor development by enhancing angiogenesis and cancer cell proliferation. In this experiment, CAMP secretion was markedly increased in cells treated with the agonist and significantly decreased in cells treated with the inhibitor. |
Xinshuo Wang, Xi’an Jiaotong University
Verified customer
Submitted 2026-02-25
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