Anti-CAND2 Antibody

Product Name

Anti-CAND2 Antibody

View all CAND2 Antibodies

SKU/Catalog Number

A10530

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-CAND2 Antibody (Catalog # A10530). Tested in IP, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store CAND2 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-CAND2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10530)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1130-1143 of Human CAND2/TIP120B (C-terminal) coupled to KLH.

Reactive Species

A10530 is reactive to CAND2 in Human, Mouse, Rat

Calculated molecular weight

135.3 kDa

Background of CAND2

Anti-CAND2 antibody is ideal for western blotting, ELISA and IHC. CAND2 is also known as TIP120B, and TATA-binding protein-interacting protein 120B. While both CAND1 (TIP120A) and CAND2 (TIP120B) are TATA-binding proteins and form complexes with various nuclear proteins involved in the control of eukaryotic gene transcription, CAND2 (TIP120B) is expressed specifically in muscle and heart tissue. This is contrary to the ubiquitous expression of CAND1 (TIP120A). TIP120 homologs exist in various higher eukaryotes including D. melanogaster, C. elegans, and A. thaliana. TIP120B is 60% identical in amino acid sequence to TIP120A.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10530 is guaranteed for ELISA, IP, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000
This antibody reacts with human, rat,  and mouse CAND2 tested by western blot and immunoprecipitation.  The antibody immunoprecipitates in vitro translated protein and protein from transfected cell lysates (using HeLa and NIH-3T3, and others).  Coimmunoprecipitation of related proteins has not been tested.  A 125.4 kDa band corresponding to human CAND2 is detected.  CAND2 is specifically expressed in muscle and heart tissue.  Researchers should determine optimal titers for other applications.

Validation Images & Assay Conditions

See full target information CAND2

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