Anti-CARD10 Antibody

Product Name

Anti-CARD10 Antibody

View all CARD10 Antibodies

SKU/Catalog Number

A07686-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-CARD10 Antibody (Catalog # A07686-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

CARD10 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-CARD10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07686-1)

Host

Rabbit

Contents

CARD10 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

CARD10 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the C-terminus of human CARD10. The immunogen is located within the last 50 amino acids of CARD10.

Reactive Species

A07686-1 is reactive to CARD10 in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

115.9 kDa

Background of CARD10

Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs, RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD-containing protein was recently identified and designated CARD10. This protein belongs to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF-κB. CARD 10 and the related protein CARMA1 also associate with NEMO, the regulatory subunit of the IκK complex, demonstrating its importance in the regulation of NF-κB transcription factor activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07686-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

CARD10 antibody can be used for detection of CARD10 by Western blot at 5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information CARD10

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