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Product Info Summary
| SKU: | A00375 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IF, IHC |
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Product info
Product Name
Anti-CD1 CD1A Monoclonal Antibody
SKU/Catalog Number
A00375
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD1 CD1A Monoclonal Antibody catalog # A00375. Tested in IHC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD1 CD1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00375)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
9H6
Isotype
IgG1
Immunogen
Synthetic Peptide of CD1
Reactive Species
A00375 is reactive to CD1A in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
37.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00375 is guaranteed for IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:50-200
IF 1:50-200
Validation Images & Assay Conditions
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Immunofluorescence analysis of Mouse-heart tissue. 1,CD1 Monoclonal Antibody (9H6) (red) was diluted at 1:200 (4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1,CD1 Monoclonal Antibody (9H6) was diluted at 1:200 (4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
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Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,CD1 Monoclonal Antibody (9H6) was diluted at 1:200 (4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,CD1 Monoclonal Antibody (9H6) was diluted at 1:200 (4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Specific Publications For Anti-CD1 CD1A Monoclonal Antibody (A00375)
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Customer Q&As
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14 Customer Q&As for Anti-CD1 CD1A Monoclonal Antibody
Question
We have been able to see staining in rat t-cell. Any tips? Is anti-CD1 Monoclonal antibody supposed to stain t-cell positively?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
From what I have seen in literature t-cell does express CD1A. From what I have seen in Uniprot.org, CD1A is expressed in thymus, t-cell, among other tissues. Regarding which tissues have CD1A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 3093894
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-02-24
Question
Is there a BSA free version of anti-CD1 Monoclonal antibody A00375 available?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD1 Monoclonal antibody A00375 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-31
Question
Will A00375 anti-CD1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
As indicated on the product datasheet, A00375 anti-CD1 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-01-15
Question
We are currently using anti-CD1 Monoclonal antibody A00375 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The anti-CD1 Monoclonal antibody (A00375) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-19
Question
Is a blocking peptide available for product anti-CD1 Monoclonal antibody (A00375)?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
We do provide the blocking peptide for product anti-CD1 Monoclonal antibody (A00375). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-16
Question
Does anti-CD1 Monoclonal antibody A00375 work for IHC with thymus?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
According to the expression profile of thymus, CD1A is highly expressed in thymus. So, it is likely that anti-CD1 Monoclonal antibody A00375 will work for IHC with thymus.
Boster Scientific Support
Answered: 2019-05-24
Question
We need to test anti-CD1 Monoclonal antibody A00375 on mouse thymus for research purposes, then I may be interested in using anti-CD1 Monoclonal antibody A00375 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
The products we sell, including anti-CD1 Monoclonal antibody A00375, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-15
Question
Here is the WB image, lot number and protocol we used for thymus using anti-CD1 Monoclonal antibody A00375. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-11-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-08
Question
I see that the anti-CD1 Monoclonal antibody A00375 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-08-27
Question
My question regarding product A00375, anti-CD1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00375 anti-CD1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-24
Question
I was wanting to use your anti-CD1 Monoclonal antibody for IHC for mouse thymus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse thymus identification?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
As indicated on the product datasheet, A00375 anti-CD1 Monoclonal antibody has been tested for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse thymus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thymus using anti-CD1 Monoclonal antibody A00375. Let me know if you need anything else.
K. Li
Verified customer
Asked: 2017-08-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-24
Question
My team were satisfied with the WB result of your anti-CD1 Monoclonal antibody. However we have observed positive staining in thymus cell membrane using this antibody. Is that expected? Could you tell me where is CD1A supposed to be expressed?
D. Krishna
Verified customer
Asked: 2014-08-04
Answer
From literature, thymus does express CD1A. Generally CD1A expresses in cell membrane. Regarding which tissues have CD1A expression, here are a few articles citing expression in various tissues:
T-cell, Pubmed ID: 3093894
Thymus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2014-08-04
Question
Is this A00375 anti-CD1 Monoclonal antibody reactive to the isotypes of CD1A?
B. Moore
Verified customer
Asked: 2013-08-02
Answer
The immunogen of A00375 anti-CD1 Monoclonal antibody is Synthetic Peptide. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-08-02
