Anti-Estrogen Receptor/ESR1 Antibody Picoband®

Western blot analysis of Estrogen Receptor using anti-Estrogen Receptor antibody (PB9192).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human T-47D whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Estrogen Receptor antigen affinity purified polyclonal antibody (Catalog # PB9192) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Estrogen Receptor at approximately 66 kDa. The expected band size for Estrogen Receptor is at 66 kDa.

Immunohistochemical stain of HEV ORF2 and ORF3 antigen. ( A – C ) Expression of HEV ORF2 in ovarian tissues, no positive signals in ovaries from control group (A). The positive signals distribute in the sperm cell and ovum cytoplasm of HEV-positive ovarian section at 28 dpi (B) and 49 dpi (C). ( D – E ) Expression of HEV ORF3 in ovarian tissues, no positive signals in ovaries from control group (D). The positive signals distribute in the sperm cell and ovum cytoplasm of HEV-positive ovarian section at 28 dpi (E) and 49 dpi ( F ). (Magnification: 20×).
Index in PubMed under a CC BY license. PMID: 29435117

Histopathological analyze of ovaries. ( A ) and ( D ), There were no gross histopathological lesions in the ovarian section from the control group at 28 dpi and 49 dpi, respectively.(Magnification: 10×); ( B ) ovarian interstitial cell scattered necrosis (black arrow), a small amount of lymphocytic infiltration in part area of ovarian interstitial from HEV RNA positive rabbit at 28 dpi.(Magnification:10×); ( C ) Necrosis and scattered lymphocytic infiltration (black arrow) in part area of ovarian interstitial from HEV RNA positive rabbit at 28 dpi.(Magnification:40×); ( D ) high power field of the control group (Magnification:20×); ( E ) Large amounts of ovarian germ cell were necrosis (black arrow) and dropped off, an increase of ovarian atresia and lymphpcytic infiltration in the ovarian interstitial from the HEV RNA positive rabbit at 48 dpi. (Magnification:10×); ( F ) Large amounts of lymphcytic infiltration (green arrow) in the ovarian interstitial from the HEV RNA positive rabbit. (Magnification:40×).
Index in PubMed under a CC BY license. PMID: 29435117

Representative TUNEL-stained histological sections of ovaries from control group and Swine HEV inoculated rabbits. TUNEL-positive cells have brown nuclei. The number of TUNEL-positive cells were remarkably higher in HEV RNA positive ovaries at 28dpi ( B ) and 49dpi ( C ) than in the control ( A ). (magnification:40×). Quantitative analysis of TUNEL-positive cells in ovaries of rabbits ( D ). The data were expressed as the percentage (mean ± SD). ( * P < 0.05) or ( ** P < 0.01), indicated statistical significance versus the control group ( n = 5).
Index in PubMed under a CC BY license. PMID: 29435117

Expression level of protein of Bcl-2, Bax and Caspase-3 in ovary tissue of different groups at 28 dpi and 49 dpi. ( A ) intensity of expression of Bcl-2 ( B ) Bax, ( C ) Caspase-3 in ovaries of HEV inoculation rabbits. ( D ) Western blot analysis of Bcl-2, Bax and Caspase-3 in ovaries in different groups.
Index in PubMed under a CC BY license. PMID: 29435117

IHC analysis of Estrogen Receptor using anti-Estrogen Receptor antibody (PB9192).
Estrogen Receptor was detected in a paraffin-embedded section of human mammry cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Estrogen Receptor Antibody (PB9192) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.