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Product Info Summary
| SKU: | A04432 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD116 CSF2RA Antibody
SKU/Catalog Number
A04432
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD116 CSF2RA Antibody catalog # A04432. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD116 CSF2RA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04432)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CSF2RA. AA range:121-170
Reactive Species
A04432 is reactive to CSF2RA in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
46.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04432 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-lymph, antibody was diluted at 1:100
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Western Blot analysis of L929 cells using CD116 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from L929 cells, using CSF2RA Antibody.
Specific Publications For Anti-CD116 CSF2RA Antibody (A04432)
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-CD116 CSF2RA Antibody
Question
Does A04432 anti-CD116 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
As indicated on the product datasheet, A04432 anti-CD116 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-30
Question
Here is the WB image, lot number and protocol we used for placenta using anti-CD116 antibody A04432. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-13
Question
I was wanting to use your anti-CD116 antibody for WB for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2020-01-20
Answer
It shows on the product datasheet, A04432 anti-CD116 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-20
Question
Would anti-CD116 antibody A04432 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
According to the expression profile of placenta, CSF2RA is highly expressed in placenta. So, it is likely that anti-CD116 antibody A04432 will work for WB with placenta.
Boster Scientific Support
Answered: 2019-11-06
Question
Is a blocking peptide available for product anti-CD116 antibody (A04432)?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
We do provide the blocking peptide for product anti-CD116 antibody (A04432). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-10-29
Question
Would anti-CD116 antibody A04432 work on pig WB with blood?
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Our lab technicians have not tested anti-CD116 antibody A04432 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CD116 antibody A04432 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-06
Question
Our lab want to know about to test anti-CD116 antibody A04432 on human placenta for research purposes, then I may be interested in using anti-CD116 antibody A04432 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The products we sell, including anti-CD116 antibody A04432, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-23
Question
I see that the anti-CD116 antibody A04432 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-14
Question
Can you help my question with product A04432, anti-CD116 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
F. Jones
Verified customer
Asked: 2018-06-29
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04432 anti-CD116 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-29
Question
Is this A04432 anti-CD116 antibody reactive to the isotypes of CSF2RA?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
The immunogen of A04432 anti-CD116 antibody is Synthesized peptide derived from the Internal region of human CD116. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-04-05
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-CD116 antibody A04432. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-23
Question
Do you have a BSA free version of anti-CD116 antibody A04432 available?
Verified Customer
Verified customer
Asked: 2017-10-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD116 antibody A04432 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-24
Question
We are currently using anti-CD116 antibody A04432 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?
V. Jones
Verified customer
Asked: 2013-03-04
Answer
The anti-CD116 antibody (A04432) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-04
