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Product Info Summary
| SKU: | M00154 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD19 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00154
BM4935 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD19 Rabbit Monoclonal Antibody catalog # M00154. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD19 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00154)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AOFO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD19
Reactive Species
M00154 is reactive to CD19 in Human
Observed Molecular Weight
95 kDa
Calculated molecular weight
61.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00154 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human Raji whole cell, human Daudi whole cell, human Ramos whole cell, human K562 whole cell
IHC: Human esophageal carcinoma tissue, Human Hodgkin's lymphoma tissue, Mouse skin tissue, Rat stomach tissue
Validation Images & Assay Conditions
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Western blot analysis of CD19 using anti-CD19 antibody (M00154).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Daudi whole cell lysates,
Lane 3: human Ramos whole cell lysates,
Lane 4: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified monoclonal antibody (M00154) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD19 at approximately 95 kDa. The expected band size for CD19 is at 61 kDa.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:300 dilution.
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Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma, using the Antibody at 1:1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Mouse skin, using the Antibody at 1:100 dilution.
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Immunohistochemical analysis of paraffin-embedded Rat stomach, using the Antibody at 1:100 dilution.
Specific Publications For Anti-CD19 Rabbit Monoclonal Antibody (M00154)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD19 Rabbit Monoclonal Antibody
Question
My team were happy with the WB result of your anti-CD19 Rabbit Monoclonal antibody. However we have been able to see positive staining in spleen cell membrane using this antibody. Is that expected? Could you tell me where is CD19 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Based on literature, spleen does express CD19. Generally CD19 expresses in cell membrane. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2020-02-27
Question
I see that the anti-CD19 Rabbit Monoclonal antibody M00154 works with IF, what is the protocol used to produce the result images on the product page?
S. Mangal
Verified customer
Asked: 2020-01-20
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-01-20
Question
Is a blocking peptide available for product anti-CD19 Rabbit Monoclonal antibody (M00154)?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
We do provide the blocking peptide for product anti-CD19 Rabbit Monoclonal antibody (M00154). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-16
Question
We have been able to see staining in human embryonic kidney. Any tips? Is anti-CD19 Rabbit Monoclonal antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
From literature embryonic kidney does express CD19. From Uniprot.org, CD19 is expressed in spleen, tonsil, blood, b-cell, embryonic kidney, among other tissues. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2019-12-13
Question
Do you have a BSA free version of anti-CD19 Rabbit Monoclonal antibody M00154 available?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD19 Rabbit Monoclonal antibody M00154 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-13
Question
We are currently using anti-CD19 Rabbit Monoclonal antibody M00154 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The anti-CD19 Rabbit Monoclonal antibody (M00154) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-04
Question
Does M00154 anti-CD19 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
As indicated on the product datasheet, M00154 anti-CD19 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-28
Question
Here is the WB image, lot number and protocol we used for spleen using anti-CD19 Rabbit Monoclonal antibody M00154. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-11
Question
Will anti-CD19 Rabbit Monoclonal antibody M00154 work for IF with spleen?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to the expression profile of spleen, CD19 is highly expressed in spleen. So, it is likely that anti-CD19 Rabbit Monoclonal antibody M00154 will work for IF with spleen.
Boster Scientific Support
Answered: 2019-08-26
Question
We are interested in to test anti-CD19 Rabbit Monoclonal antibody M00154 on human spleen for research purposes, then I may be interested in using anti-CD19 Rabbit Monoclonal antibody M00154 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-01-26
Answer
The products we sell, including anti-CD19 Rabbit Monoclonal antibody M00154, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-01-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-CD19 Rabbit Monoclonal antibody M00154. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-12-20
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-12-20
Question
Can you help my question with product M00154, anti-CD19 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00154 anti-CD19 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-16
Question
Is this M00154 anti-CD19 Rabbit Monoclonal antibody reactive to the isotypes of CD19?
C. Lewis
Verified customer
Asked: 2016-01-26
Answer
The immunogen of M00154 anti-CD19 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD19. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-01-26
Question
I was wanting to use your anti-CD19 Rabbit Monoclonal antibody for IF for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?
N. Parker
Verified customer
Asked: 2014-11-05
Answer
It shows on the product datasheet, M00154 anti-CD19 Rabbit Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-05
Question
We purchased anti-CD19 Rabbit Monoclonal antibody for WB on embryonic kidney in a previous experiment. I am using human, and I plan to use the antibody for IF next. I would like examining embryonic kidney as well as tonsil in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Mitchell
Verified customer
Asked: 2014-11-03
Answer
I viewed the website and datasheets of our anti-CD19 Rabbit Monoclonal antibody and it appears that M00154 has been tested on human in both WB and IF. Thus M00154 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2014-11-03
