Anti-CD3 epsilon/CD3E Antibody Picoband™

Boster Bio Anti-CD3 epsilon/CD3E Antibody Picoband™ catalog # PB9093. Tested in IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat. Cited in 7 publication(s).

Product Info Summary

SKU: PB9093
Size: 100μg/vial
Reactive Species: Chicken, Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-CD3 epsilon/CD3E Antibody Picoband™

See all CD3E products

SKU/Catalog Number

PB9093

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD3 epsilon/CD3E Antibody Picoband™ catalog # PB9093. Tested in IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Chicken, Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD3 epsilon/CD3E Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9093)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human CD3 epsilon recombinant protein (Position: D23-I207). Human CD3 epsilon shares 65% amino acid (aa) sequence identity with mouse CD3 epsilon.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9093 is reactive to CD3E in Chicken, Human, Mouse, Rat

Applications

PB9093 is guaranteed for IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, Chicken, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat
Immunocytochemistry , 0.5-1μg/ml, Human
Immunofluorescence, 2μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For CD3E (Source: Uniprot.Org, NCBI)

Uniprot ID

P07766

Gene ID

916

Gene Name

CD3E

Full Name

T-cell surface glycoprotein CD3 epsilon chain

Weight

23.147kDa

Alternative Names

CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain CD3E IMD18, T3E, TCRE CD3e molecule T-cell surface glycoprotein CD3 epsilon chain|CD3-epsilon|CD3e antigen, epsilon polypeptide (TiT3 complex)|CD3e molecule, epsilon (CD3-TCR complex)|T-cell antigen receptor complex, epsilon subunit of T3|T-cell surface antigen T3/Leu-4 epsilon chain

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD3E, check out the CD3E Infographic

CD3E infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD3E: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PB9093 has been cited in 7 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis
Species: Mouse

The pathological features of ectopic lymphoid neogenesis in idiopathic dacryoadenitis

Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients

Chen B, Liu L, Xu H, Yang Y, Zhang L, Zhang F. Exp Ther Med. 2015 Mar;9(3):1063-1067. Epub 2015 Jan 26. Effectiveness Of Immune Therapy Combined With Chemotherapy On The Immune Function And Recurrence Rate Of Cervical Cancer.

Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

Xie Zj, Jia Lm, He Yc, Gao Jt. World J Gastroenterol. 2006 Mar 21;12(11):1757-60. Morphological Observation Of Tumor Infiltrating Immunocytes In Human Rectal Cancer.

Li Y, Geng S, Yin Q, Chen S, Yang L, Wu X, Li B, Du X, Schmidt Ca, Przybylski Gk. J Transl Med. 2010 May 14;8:47. Doi: 10.1186/1479-5876-8-47. Decreased Level Of Recent Thymic Emigrants In Cd4+ And Cd8+T Cells From Cml Patients.

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6 Customer Q&As for Anti-CD3 epsilon/CD3E Antibody Picoband™

Question

Our lab used your anti-CD3 epsilon/CD3E antibody for WB on blood in a previous experiment. I am using mouse, and We want to use the antibody for IHC-P next. We are interested in examining blood as well as thymus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

I looked at the website and datasheets of our anti-CD3 epsilon/CD3E antibody and it seems that PB9093 has been tested on mouse in both WB and IHC-P. Thus PB9093 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-04-09

Question

We are currently using anti-CD3 epsilon/CD3E antibody PB9093 for mouse tissue, and we are content with the IHC-F results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

The anti-CD3 epsilon/CD3E antibody (PB9093) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-13

Question

We were content with the WB result of your anti-CD3 epsilon/CD3E antibody. However we have seen positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is CD3E supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-03-11

Answer

From what I have seen in literature, leukemic t-cell does express CD3E. Generally CD3E expresses in cell membrane. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-03-11

Question

We are currently using anti-CD3 epsilon/CD3E antibody PB9093 for mouse tissue, and we are content with the IHC-F results. The species of reactivity given in the datasheet says chicken, human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

The anti-CD3 epsilon/CD3E antibody (PB9093) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-28

Question

We have observed staining in chicken leukocyte. What should we do? Is anti-CD3 epsilon/CD3E antibody supposed to stain leukocyte positively?

Verified Customer

Verified customer

Asked: 2018-02-12

Answer

From what I have seen in literature leukocyte does express CD3E. From what I have seen in Uniprot.org, CD3E is expressed in leukocyte, blood, thymus, leukemic t-cell, among other tissues. Regarding which tissues have CD3E expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3267235, 15489334
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Thymus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-02-12

Question

I would like using your anti-CD3 epsilon/CD3E antibody for pd-1 signaling studies. Has this antibody been tested with western blotting on jurkat whole cell lysate? We would like to see some validation images before ordering.

J. Zhao

Verified customer

Asked: 2014-09-01

Answer

Thank you for your inquiry. This PB9093 anti-CD3 epsilon/CD3E antibody is tested on jurkat whole cell lysate, cem whole cell lysate. It is guaranteed to work for IF, IHC-P, IHC-F, ICC, WB in chicken, human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-09-01

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