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- Table of Contents
GP9 antibody (A04595-2) performs well in IHC, WB and shows reactivity with Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IHC analysis of CD42a/GP9 using anti-CD42a/GP9 antibody (A04595-2).
CD42a/GP9 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD42a/GP9 Antibody (A04595-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
CD42a/GP9 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD42a/GP9 Antibody (A04595-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Anti-CD42a/GP9 Antibody Picoband®
GP9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
GP9 antibody was positively detected by WB in:
rat spleen tissue, rat lung tissue, model group-mouse hippocampus tissue, Drug treatment (/kg) ÿ mouse hippocampus tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GP9 antibody was positively detected by IHC in:
mouse spleen tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Mouse
Tested
Recommend
Dog
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD42a/GP9 Antibody Picoband®
View all GP9 Antibodies
View all GP9 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse CD42a/GP9. Mouse CD42a/GP9 shares 89.5% amino acid (aa) sequence identity with human CD42a/GP9.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
19 kDa
Calculated Molecular Weight
19.7 kDa
Background of GP9
Involved in blood coagulation and megakaryocyte development. Predicted to be part of glycoprotein Ib-IX-V complex. Is expressed in several structures, including brain; foregut; hemolymphoid system; liver; and male reproductive gland or organ. Human ortholog(s) of this gene implicated in Bernard-Soulier syndrome. Orthologous to human GP9 (glycoprotein IX platelet).
Cite This Product
Anti-CD42a/GP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04595-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Mouse
100%
High Homology
Rat
100%
High Homology
Dog
95%
High Homology
Human
89%
Moderate Homology
Monkey
89%
Moderate Homology
Rabbit
84%
Moderate Homology
Cow
74%
Moderate Homology
Sheep
74%
Moderate Homology
Zebrafish
47%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GP9 antibody
Immunohistochemistry (IHC) protocol for GP9 antibody
Review
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1 Review For Anti-CD42a/GP9 Antibody Picoband®
Western blot analysis was performed using the GP9 antibody to detect GP9 protein expression in the mouse hippocampus. This antibody is highly efficient and specific, making it suitable for quantitative WB detection of GP9 in mouse tissues.
| SKU | M00024-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampus tissue |
| Sample Processing Description | The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE. |
| Primary Antibody | Anti-AKT1 Monoclonal Antibody |
| Primary Incubation | overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon |
| Results Summary | CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear and free of non-specific signals. After recovery, the antibody could be reused and still showed good performance. |
Changbin Yuan, LNUTCM
Verified customer
Submitted 2025-11-06
Product Promise
We promise Anti-CD42a/GP9 Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A04595-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026