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Product Info Summary
| SKU: | A04595-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD42a/GP9 Antibody Picoband®
SKU/Catalog Number
A04595-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD42a/GP9 Antibody Picoband® catalog # A04595-2. Tested in WB, IHC applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD42a/GP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04595-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of mouse CD42a/GP9. Mouse CD42a/GP9 shares 89.5% amino acid (aa) sequence identity with human CD42a/GP9.
Reactive Species
A04595-2 is reactive to GP9 in Mouse, Rat
Observed Molecular Weight
19 kDa
Calculated molecular weight
19.7 kDa
Background of GP9
Involved in blood coagulation and megakaryocyte development. Predicted to be part of glycoprotein Ib-IX-V complex. Is expressed in several structures, including brain; foregut; hemolymphoid system; liver; and male reproductive gland or organ. Human ortholog(s) of this gene implicated in Bernard-Soulier syndrome. Orthologous to human GP9 (glycoprotein IX platelet).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04595-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse
Validation Images & Assay Conditions
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Western blot analysis of CD42a/GP9 using anti-CD42a/GP9 antibody (A04595-2).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD42a/GP9 antigen affinity purified polyclonal antibody (A04595-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for CD42a/GP9 at approximately 19 kDa. The expected band size for CD42a/GP9 is at 19 kDa.
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IHC analysis of CD42a/GP9 using anti-CD42a/GP9 antibody (A04595-2).
CD42a/GP9 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD42a/GP9 Antibody (A04595-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Western blot analysis of GP9 using anti-GP9 antibody (A04595-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: control group-Mouse hippocampus tissue lysates,
Lane 2: model group-Mouse hippocampus tissue lysates,
Lane 3: Drug treatment (0.1g/kg) – Mouse hippocampus tissue lysates,
,
Lane 4: Drug treatment (0.5g/kg) – Mouse hippocampus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GP9 antigen affinity purified polyclonal antibody (A04595-2) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with ChemiDoc MP system. A specific band was detected for GP9 at approximately 19 kDa. The expected band size for GP9 is at 19 kDa.
Specific Publications For Anti-CD42a/GP9 Antibody Picoband® (A04595-2)
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Customer Reviews
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1 Reviews For Anti-CD42a/GP9 Antibody Picoband®
Western blot analysis was performed using the GP9 antibody to detect GP9 protein expression in the mouse hippocampus. This antibody is highly efficient and specific, making it suitable for quantitative WB detection of GP9 in mouse tissues.
Excellent
| SKU | M00024-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampus tissue |
| Sample Processing Description | The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE. |
| Primary Antibody | Anti-AKT1 Monoclonal Antibody |
| Primary Incubation | overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon |
| Results Summary | CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear and free of non-specific signals. After recovery, the antibody could be reused and still showed good performance. |
Changbin Yuan, LNUTCM
Verified customer
Submitted 2025-11-06
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