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Product Info Summary
| SKU: | M00360 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-CD47 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00360
BM4131 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD47 Rabbit Monoclonal Antibody catalog # M00360. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD47 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00360)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
D-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD47
Reactive Species
M00360 is reactive to CD47 in Human, Mouse, Rat
Observed Molecular Weight
54 kDa
Calculated molecular weight
35.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00360 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of extracts of NIH/3T3 cell lysate, using CD47 antibody.
Specific Publications For Anti-CD47 Rabbit Monoclonal Antibody (M00360)
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4 Customer Q&As for Anti-CD47 Rabbit Monoclonal Antibody
Question
We are currently using anti-CD47 Rabbit Monoclonal antibody M00360 for mouse tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
D. Banerjee
Verified customer
Asked: 2020-05-01
Answer
The anti-CD47 Rabbit Monoclonal antibody (M00360) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-05-01
Question
We have tried in the past anti-CD47 Rabbit Monoclonal antibody for IF on visceral pleura a few years ago. I am using mouse, and We are going to use the antibody for WB next. My lab would like examining visceral pleura as well as hippocampus in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
T. Banerjee
Verified customer
Asked: 2020-01-24
Answer
I took a look at the website and datasheets of our anti-CD47 Rabbit Monoclonal antibody and I see that M00360 has been validated on mouse in both IF and WB. Thus M00360 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-24
Question
My boss were happy with the WB result of your anti-CD47 Rabbit Monoclonal antibody. However we have observed positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is CD47 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
Based on literature, leukemic t-cell does express CD47. Generally CD47 expresses in cell membrane. Regarding which tissues have CD47 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Erythrocyte, Pubmed ID: 7998989
Hippocampus, Leiomyosarcoma, and Ovarian adenocarcinoma, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Myelomonocyte, Pubmed ID: 7691831
Ovary, Pubmed ID: 1394148
T-cell, Pubmed ID: 15383453
Boster Scientific Support
Answered: 2019-11-12
Question
We have seen staining in mouse brain. Are there any suggestions? Is anti-CD47 Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
From what I have seen in literature brain does express CD47. From what I have seen in Uniprot.org, CD47 is expressed in visceral pleura, ovary, myelomonocyte, brain, hippocampus, leiomyosarcoma ovarian adenocarcinoma, erythrocyte, t-cell, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD47 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14702039
Erythrocyte, Pubmed ID: 7998989
Hippocampus, Leiomyosarcoma, and Ovarian adenocarcinoma, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Liver, Pubmed ID: 19159218
Myelomonocyte, Pubmed ID: 7691831
Ovary, Pubmed ID: 1394148
T-cell, Pubmed ID: 15383453
Boster Scientific Support
Answered: 2019-08-12
