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CD68 antibody (A00602-1) performs well in ELISA, IF, IHC and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IF analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-CD68 Antibody
CD68 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, IHC |
Tested Applications
CD68 antibody was positively detected by IHC in:
human tonsil tissue, human spleen tissue, human liver cancer, human prostate cancer, human breast cancer
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CD68 antibody was positively detected by IF in:
human tonsil tissue, human prostate cancer tissue
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
ELISA
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-CD68 Antibody
View all CD68 Antibodies
View all CD68 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD68 recombinant protein (Position: K154-L354).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated Molecular Weight
37.4 kDa
Background of CD68
CD68, cluster of differentiation, is a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. CD68 is a member of a family of hematopoietic mucin-like molecules that includes leukosialin/CD43 and stem cell antigen CD34. The CD68 gene is mapped to 17p13.1. Immunohistochemistry can be used to identify the presence of CD68, which is found in the cytoplasmic granules of a range of different blood cells. It is particularly useful as a marker for the various cells of the macrophage lineage, including monocytes, histiocytes, giant cells, Kupffer cells, and osteoclasts. This allows it to be used to distinguish diseases of otherwise similar appearance, such as the monocyte/macrophage and lymphoid forms of leukaemia (the latter being CD68 negative). Its presence in macrophages also makes it useful in diagnosing conditions related to proliferation or abnormality of these cells, such as malignant histiocytosis, histiocytic lymphoma, and Gaucher's disease.
Cite This Product
Anti-CD68 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00602-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Rabbit
81%
Moderate Homology
Cow
79%
Moderate Homology
Sheep
78%
Moderate Homology
Mouse
77%
Moderate Homology
Rat
76%
Moderate Homology
Dog
72%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Immunohistochemistry (IHC) protocol for CD68 antibody
Immunofluorescence (IF) protocol for CD68 antibody
Publications
Application:
Species:
Orchestration of hepatocyte inflammatory responses by monocytes during acute viral hepatitis in ducks in vitro
Published: 2025-10-17
Journal: VETERINARY RESEARCH
Application: IF
Impact Factor: 3.50
Species: Duck
PMID: 41108011
Tauroursodeoxycholic acid regulates macrophage/monocyte distribution and improves spinal microenvironment to promote nerve regeneration through inhibiting NF-κB signaling pathway in spinal cord injury
Published: 2025-04-10
Journal: Frontiers in Pharmacology
Application: IF
Impact Factor: 4.80
Species: Mouse
PMID: 40276612
Clinical Significance of M1/M2 Macrophages and Related Cytokines in Patients with Spinal Tuberculosis
Published: 2020-05-25
Journal: DISEASE MARKERS
Application: IHC
Impact Factor: 2.74
Species: Human
PMID: 32566036
Elevated Levels of TNF-α and Decreased Levels of CD68-Positive Macrophages in Primary Tumor Tissues Are Unfavorable for the Survival of Patients With Nasopharyngeal Carcinoma.
Published: 2019-01-01
Journal: TECHNOLOGY IN CANCER RESEARCH & TREATMENT
Application: IHC
Impact Factor: 1.48
Species: Human
PMID: 31522611
The up-regulation of endothelin-1 and down-regulation of miRNA-125a-5p, -155, and -199a/b-3p in human atherosclerotic coronary artery
Published: 2014-04-05
Journal: CARDIOVASCULAR PATHOLOGY
Application: IF
Impact Factor: 2.34
Species: Human
PMID: 24877885
Review
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1 Review For Anti-CD68 Antibody
Immunofluorescence analysis using the Anti-CD68 antibody (A00602-1) on human normal and preterm placenta showed minimal CD68 expression in normal samples and abundant expression in preterm inflamed samples, consistent with expected results.
| SKU | A00602-1 |
|---|---|
| Application | Immunofluorescence |
| Sample | human normal and preterm placenta |
| Sample Processing Description | Human normal-term and preterm placentas were collected clinically and processed for longitudinal paraffin embedding, allowing visualization of both the amnion and chorion membranes. |
| Other Reagents | Goat serum, DAPI, anti-fade mounting medium |
| Primary Antibody | Anti-CD68 Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | DyLight 594-conjugated Donkey Anti-Mouse IgG (H+L)（BA1148） |
| Secondary Incubation | 1:500, 45 min in 37℃ |
| Detection | Image system:Leica DM2500 |
| Results Summary | In normal placentas, macrophages are scarce or nearly absent, showing only sporadic CD68 expression, whereas in preterm placentas with inflammation, macrophages are abundant and CD68 expression is markedly increased, consistent with expected theoretical data. |
Yuan Wang, Shandong First Medical University
Verified customer
Submitted 2026-02-27
Product Promise
We promise Anti-CD68 Antibody performs excellently in relevant applications for ELISA, IF, IHC. If it doesn't perform as promised, we will refund the product. Learn more
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A00602-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026