Anti-CD68 Antibody

IHC analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IHC analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/ml rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. HRP-AffiniPure Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

IF analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. DyLight 488 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human normal placenta and preterm placentar tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:500 rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. DyLight 594-conjugated Donkey Anti-Mouse IgG (H+L)(BA1148) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of CD68 using anti-CD68 antibody (A00602-1).
CD68 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2.5 μg/mL rabbit anti-CD68 Antibody (A00602-1) overnight at 4°C. DyLight 488 Conjugated AffiniPure Goat Anti-rabbit IgG (H+L) (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Coordination of the hepatocyte IL-1β transcription-translation-mature tRNAome axis by monocytes . A Representative images of monocytes (light microscopy and hematoxylin staining). The identification of monocytes was confirmed by a rabbit anti-CD68 polyclonal antibody (green), and the results were compared with those of DEFs. DAPI was used for nuclear staining. B The impact of monocytes on the transcription of proinflammatory cytokines in infected hepatocytes. The hepatocytes were cocultured with a total of 5.0 × 10 6 PMBCs per well or with the corresponding monocytes derived from the same number of PBMCs. A total of 2.0 × 10 7 copies per well of DHAV were subsequently used. Samples were collected at 24 hpc. RT‒qPCR was used to quantify the expression of IL-1β, IL-6, and TNF-α compared with that in uninfected DPHs ( n = 3). Statistical significance was determined via unpaired t tests; *, p < 0.05; **, p < 0.01; ***, p < 0.001. C The impact of PBMCs or monocytes on the 23 selected tRNAs in infected DPHs. The tRNA data were normalized to those of the uninfected DPHs ( n = 3). D Western blotting of the IL-1β protein in infected DPHs cocultured with PBMCs or monocytes at 24 hpc, with the same number of cells used as in panel B. The gray value of the IL-1β protein was then quantitatively evaluated via ImageJ software. E Dynamic changes in the expression of IL-1β mRNA in DHAV-infected DPHs cocultured with monocytes from 12 to 48 hpc ( n = 3). DHAV-infected DPHs lacking monocyte coculture were used as a control, and uninfected DPHs were used as a negative control for gene normalization. F Dynamic changes in the 23 selected tRNAs in DPHs cocultured with monocytes. The tRNA data were normalized to those of the uninfected DPHs ( n = 3). G Western blotting of IL-1β in infected DPHs during different durations of coculture with monocytes (12 hpc, 24 hpc, and 48 hpc). The amount of IL-1β protein was determined by analysing the gray value via ImageJ software.
Index in PubMed under a CC BY license. PMID: 41108011

TUDCA promoted macrophages shift to M2-like phenotype and impacted endogenous NSCs morphology. (A) Immunofluorescent staining of M1-like (iNOS, red) and M2-like macrophages (CD163, green) at the margin of the lesion site at day 7 after SCI. (B, C) Quantification the number of iNOS + or CD163 cells. (D) Macrophages (CD68, red) and endogenous NSCs (Nestin, green) at the margin of the lesion site at day 7 after SCI. All experiments were performed in triplicated and data were presented means ± SEM, n = 3 per group. **P < 0.01, ***P < 0.001 .
Index in PubMed under a CC BY license. PMID: 40276612

TUDCA regulated macrophages and reactive astrocytes distribution. Co-immunofluorescence images showed macrophages (CD68, red) and reactive astrocytes (GFAP, green) at day 3 and day 7 after SCI.
Index in PubMed under a CC BY license. PMID: 40276612

TUDCA regulated macrophages and endogenous NSCs distribution. Co-immunofluorescence images showed macrophages (CD68, red) and endogenous NSCs (Nestin, green) at day 3 and day 7 after SCI.
Index in PubMed under a CC BY license. PMID: 40276612