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- Table of Contents
GJA1 antibody (PA1026) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 13 Publications
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IF analysis of GJA1 using anti-GJA1 antibody (PA1026).
GJA1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
GJA1 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GJA1 Antibody (PA1026) overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Connexin 43/GJA1 Antibody Picoband®
GJA1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, WB |
Tested Applications
GJA1 antibody was positively detected by WB in:
rat heart tissue, rat brain tissue, rat H9C2 cells whole cell, mouse heart tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GJA1 antibody was positively detected by IHC in:
human heart tissue, rat heart tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GJA1 antibody was positively detected by IF in:
rat heart tissue
Recommend Dilution
Application
Dilution
Species
Immunofluorescence
2μg/ml
Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Tested
Mouse
Tested
Human
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Connexin 43/GJA1 Antibody Picoband®
View all GJA1 Antibodies
View all GJA1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated Molecular Weight
43.0 kDa
Background of GJA1
Connexins 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
Cite This Product
Anti-Connexin 43/GJA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1026)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
76%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GJA1 antibody
Immunohistochemistry (IHC) protocol for GJA1 antibody
Immunofluorescence (IF) protocol for GJA1 antibody
Publications
Application:
Species:
An anisotropic cardiac patch with barbed microneedles for enhanced tissue anchorage and myocardial repair
Published: 2025-08-30
Journal: Acta Biomaterialia
Application: IF
Impact Factor: 9.60
Species: Rat
PMID: 40889669
Ultrasmall PtIr Bimetallic Nanozyme Treats Myocardial Infarction via Ischemic/Inflammatory Cardiac Microenvironment Remodeling
Published: 2025-04-02
Journal: ACS Nano
Application: IHC
Impact Factor: 16.00
Species: Rat
PMID: 40175295
Secretome of EMSCs neutralizes LPS‑induced acute lung injury via aerosol administration
Published: 2023-09-22
Journal: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
Application: IF
Impact Factor: 5.40
Species: Rat
PMID: 37772372
Polystyrene microplastics induce blood–testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats
Published: 2021-04-25
Journal: ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
Application: WB
Impact Factor: 4.22
Species: Rat
PMID: 33895957
Granulocyte colony-stimulating factor attenuates myocardial remodeling and ventricular arrhythmia susceptibility via the JAK2-STAT3 pathway in a rabbit model of coronary microembolization
Published: 2020-02-17
Journal: BMC Cardiovascular Disorders
Application: WB
Impact Factor: 2.08
Species: Rabbit
PMID: 32066388
Involvement of sphingosine-1-phosphate receptors 2/3 in IR-induced sudden cardiac death
Published: 2019-01-02
Journal: HEART AND VESSELS
Application: IHC
Impact Factor: 1.62
Species: Rat
PMID: 30604190
Perineurium-like sheath derived from long-term surviving mesenchymal stem cells confers nerve protection to the injured spinal cord
Published: 2018-01-11
Journal: BIOMATERIALS
Application: IF
Impact Factor: 8.81
Species: Rat
PMID: 29353106
Efficacy of Bushenjianpi prescription on autoimmune premature ovarian failure in mice
Published: 2017-12-06
Journal: Journal of Traditional Chinese Medicine
Application: IHC,WB
Impact Factor: 0.99
Species: Mouse
Rehmannia glutinosa oligosaccharide induces differentiation of bone marrow mesenchymal stem cells into cardiomyocyte-like cells
Published: 2016-10-17
Journal: GENETICS AND MOLECULAR RESEARCH
Application: WB
Impact Factor: 0.76
Species: Rat
PMID: 27813551
Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats
Published: 2016-08-30
Journal: Bioengineered
Application: WB
Impact Factor: 1.87
Species: Rat
PMID: 27575818
Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
Published: 2016-01-18
Journal: ACTA PHARMACOLOGICA SINICA
Application: WB
Impact Factor: 3.17
Species: Rat
PMID: 26775665
Effects of ischemic preconditioning on ischemia/reperfusion-induced arrhythmias by upregulatation of connexin 43 expression
Published: 2011-06-02
Journal: Journal of Cardiothoracic Surgery
Application: IF
Impact Factor: 0.91
Species: Rabbit
PMID: 21635761
Blockade of angiotensin II improves hyperthyroid induced abnormal atrial electrophysiological properties
Published: 2011-05-03
Journal: REGULATORY PEPTIDES
Application: WB
Impact Factor: 2.47
Species: Rabbit
PMID: 21545815
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Product Promise
We promise Anti-Connexin 43/GJA1 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1026
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 6, 2026