Product Info Summary
SKU: | A09292-2 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD72 Antibody Picoband™
SKU/Catalog Number
A09292-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD72 Antibody Picoband™ catalog # A09292-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD72 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09292-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD72 recombinant protein (Position: A8-R346).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09292-2 is reactive to CD72 in Mouse, Rat
Applications
A09292-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
35 kDa
Calculated molecular weight
40.22kDa
Background of Cd72
CD72 (Cluster of Differentiation 72), also known in murine biology as Lyb-2, is a protein active in the immune systemof animals. This gene is mapped to 9p13.3. It consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. It is a ligand for CD5.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
Flow Cytometry, 1-3μg/1x106 cells, Mouse
Direct ELISA, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD72 using anti-CD72 antibody (A09292-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: mouse liver tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD72 antigen affinity purified polyclonal antibody (Catalog # A09292-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD72 at approximately 35KD. The expected band size for CD72 is at 35KD.
Click image to see more details
Figure 2. IHC analysis of CD72 using anti-CD72 antibody (A09292-2).
CD72 was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD72 Antibody (A09292-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD72 using anti-CD72 antibody (A09292-2).
CD72 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD72 Antibody (A09292-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. Flow Cytometry analysis of ANA-1 cells using anti-CD72 antibody (A09292-2).
Overlay histogram showing ANA-1 cells stained with A09292-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD72 Antibody (A09292-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 5. Flow Cytometry analysis of mouse spleen tissue using anti-CD72 antibody (A09292-2).
Overlay histogram showing mouse spleen tissue stained with A09292-2 (Blue line).The tissue was blocked with 10% normal goat serum. And then incubated with rabbit anti-CD72 Antibody (A09292-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 6. IF analysis of CD72 using anti-CD72 antibody (A09292-2).
CD72 was detected in immunocytochemical section of HEPA1-6 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CD72 Antibody (A09292-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CD72 (Source: Uniprot.org, NCBI)
Gene Name
CD72
Full Name
B-cell differentiation antigen CD72
Weight
40.22kDa
Alternative Names
CD72 antigenB-cell differentiation antigen CD72; CD72 molecule; CD72; CD72b; Ly-19.2; Ly-32.2; Lyb-2; LYB2lyb-2 CD72 CD72b, LYB2 CD72 molecule B-cell differentiation antigen CD72|CD72 antigen|lyb-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD72, check out the CD72 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD72: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD72 Antibody Picoband™ (A09292-2)
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