Anti-CD73 Antibody (Monoclonal, ABM40E2)

Product Name

Anti-CD73 Antibody (Monoclonal, ABM40E2)

View all NT5E Antibodies

SKU/Catalog Number

M02120

Size

100 µg

Form

Liquid

Description

Boster Bio Anti-CD73 Antibody (Monoclonal, ABM40E2) (Catalog# M02120). Tested in IHC, FACS, WB application(s). This antibody reacts with Human.

Storage & Handling

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Cite This Product

Anti-CD73 Antibody (Monoclonal, ABM40E2) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02120)

Host

Mouse

Contents

100 µg in 200 µl PBS containing 0.05% stabilizing protein and 0.05% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

ABM40E2

Isotype

IgG2a Kappa

Immunogen

A partial length recombinant CD73 protein (amino acids 145-346)

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M02120 is reactive to NT5E in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

63.4 kDa

Background of NT5E

The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. This monoclonal antibody recognizes a protein of 83kDa, which is identified as Factor XIIIa. It has been identified in platelets, megakaryocytes, and fibroblast-like mesenchymal or histiocytic cells in the placenta, uterus, and prostate, monocytes and macrophages and dermal dendritic cells. Anti-factor XIIIa has been found to be useful in differentiating between dermatofibroma (almost all cases are positive), dermatofibrosarcoma protuberans (-/+) and desmoplastic malignant melanoma (-). Anti-factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02120 is guaranteed for IHC, WB, FACS Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot: 2-4 µg/ml
IHC: 5 µg/ml, FACS: 0.2-0.5 µg/10^6 cells

Validation Images & Assay Conditions

See full target information NT5E

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