|Ig Isotype||Mouse IgG2a|
|Applications||Flow Cytometry, IHC, ICC|
|Product Name||Anti-CD8 Antibody (Monoclonal, CA-8)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CD8, CD8a molecule; CD8b molecule (CD8A; CD8B) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CD8 Antibody (Monoclonal, CA-8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1017)|
|Contents/Buffer||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Immunogen||Human thymocytes followed by peripheral blood T cells.|
Assay Dilutions Overview
Immunohistochemistry(Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
Images And Assay Conditions
Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD8 antibody (MA1017).
Overlay histogram showing human PBMC cells stained with MA1017 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD8 Antibody (MA1017,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info (Source: Uniprot.org)
|Gene Name||CD8A; CD8B|
|Protein Name||T-cell surface glycoprotein CD8 beta chain|
|Alternative Names||T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1;|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Molecular Weight||23400 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.|
|Background||The human lymphocyte differentiation antigen CD8 is encoded by a single gene that gives rise to a 33- to 34-kDa glycoprotein expressed on the cell surface as a dimer and in higher molecular mass forms.|
Other Recommended Resources
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