SKU MA1017
Size 100μg/vial
Reactivity Human
Clonality Monoclonal
Clone Number CA-8
Host Mouse
Ig Isotype Mouse IgG2a
Applications Flow Cytometry, IHC, ICC

Overview

Product Name Anti-CD8 Antibody (Monoclonal, CA-8)
SKU/Catalog Number MA1017
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Mouse IgG monoclonal antibody for CD8, CD8a molecule; CD8b molecule (CD8A; CD8B) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.
Cite This Product Anti-CD8 Antibody (Monoclonal, CA-8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1017)
Host Mouse
Immunogen Human thymocytes followed by peripheral blood T cells.
Reactivity Human

Assay Details

Assay Dilutions Overview

Immunohistochemistry(Frozen Section), 1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.

Images And Assay Conditions

/m/a/ma1017 cd8 primary antibodies fc testing 1.jpg

Figure 1. Flow Cytometry analysis of human PBMC cells using anti-CD8 antibody (MA1017).
Overlay histogram showing human PBMC cells stained with MA1017 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD8 Antibody (MA1017,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P05541
Gene Name Cd8b
Protein Name T-cell surface glycoprotein CD8 beta chain
Alternative Names T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1;
Subcellular Localization Membrane; Single-pass type I membrane protein.
Molecular Weight 23400 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.
Background The human lymphocyte differentiation antigen CD8 is encoded by a single gene that gives rise to a 33- to 34-kDa glycoprotein expressed on the cell surface as a dimer and in higher molecular mass forms.

Order Product (MA1017)

Promotion:

Buy primary get secondary antibody for free.
Option Price
30ug sample size $99
100ug $280
100ug+Free HRP Secondary BA1050 $280
100ug+Free Biotin Secondary BA1001 $280

USD $280

Ships in 5-7 business days.

Troubleshooting

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Publications

Xu Q, Chen Y, Zhao Wm, Huang Zy, Duan Xj, Tong Yy, Zhang Y, Li X, Chang Gb, Chen Gh. Mol Biol Rep. 2015 Feb;42(2):431-9. Doi: 10.1007/S11033-014-3784-3. Epub 2014 Oct 21. The Cd8?? Gene In Duck (Anatidae): Cloning, Characterization, And Expression ...
Zhao N, Li X, He X, Qiu Y, Zhu L, Qi F. J Gene Med. 2013 Oct;15(10):366-74. Doi: 10.1002/Jgm.2739. Interleukin-15 Gene Therapy And The Mammalian Target Of Rapamycin Inhibitor Everolimus Inhibit The Growth Of Metastatic Breast Cancer.
Wang X, Shi L, Tu Q, Wang H, Zhang H, Wang P, Zhang L, Huang Z, Zhao F, Luan H, Wang X. Int J Nanomedicine. 2015 Jan 6;10:347-55. Doi: 10.2147/Ijn.S71245. Ecollection 2015. Treating Cutaneous Squamous Cell Carcinoma Using 5-Aminolevulinic Acid Pol...
Xu Y, Tan X, Wang D, Wang W, Li Y, Wu M, Chen S, Wu Y, Tan C. Int J Mol Med. 2015 Jun;35(6):1501-10. Doi: 10.3892/Ijmm.2015.2154. Epub 2015 Mar 24. Elevated Survivin Expression In Peripheral Blood Mononuclear Cells Is Central To Collateral Formati...
Wu Y, Wang Y, Qi X, Zhang P, Zhang C, Zhang W. Nephron Exp Nephrol. 2014;128(1-2):46-56. Doi: 10.1159/000366446. Epub 2014 Nov 5. Increased Macrophage Activation Inhibited By Tacrolimus In The Kidney Of Diabetic Rats.

Customer Q&As

Q: What would be the approximate optimal dilution for this antibody? Can we also use this antibody on paraffin embedded sections? Keyword: protocol, applications
A: The antibodies are lyophilized, and the recommended working concentration is 1μg/ml. This antibody can only be used on frozen section.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is cd86 antibody