|Application:||Flow Cytometry, IHC, ICC|
Data & Images
|Product Name||Anti-CD8 Antibody (Monoclonal, CA-8)|
|Description||Mouse IgG monoclonal antibody for CD8, CD8a molecule; CD8b molecule (CD8A; CD8B) detection. Tested with IHC-F, ICC, FCM in Human. No cross reactivity with other proteins.|
|Cite This Product||Anti-CD8 Antibody (Monoclonal, CA-8) CA-8 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1017)|
|Replacement Item||This antibody may replace the following items: sc-1141|sc-1143|sc-1144|sc-1177|sc-1181|sc-19994|sc-25277|sc-51735|sc-53212|sc-70791|sc-70794|sc-7188|sc-7970|sc-9147 from Santa Cruz Biotechnology.|
|Application||Flow Cytometry, IHC, ICC
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Human thymocytes followed by peripheral blood T cells.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain|
|Molecular Weight||23400 MW|
|Protein Function||Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing.|
|Sequence Similarities||Contains 1 Ig-like V-type (immunoglobulin-like) domain.|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Alternative Names||T-cell surface glycoprotein CD8 beta chain;CD8 antigen 37 kDa chain;OX-8 membrane antigen;CD8b;Cd8b;Cd8b1;|
Background for T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain
Anti-CD8 Antibody (Monoclonal, CA-8) Images
Click the images to enlarge.
Overlay histogram showing human PBMC cells stained with MA1017 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CD8 Antibody (MA1017,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.