Anti-BCAT1 Antibody

BCAT1 antibody

Boster Bio Anti-BCAT1 Antibody catalog # PA1538. Tested in IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: PA1538
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-BCAT1 Antibody

View all BCAT1 Antibodies

SKU/Catalog Number

PA1538

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BCAT1 Antibody catalog # PA1538. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BCAT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1538)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human BCAT1, different from the related mouse sequence by one amino acid, rat sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1538 is reactive to BCAT1 in Human

Applications

PA1538 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

43 kDa

Calculated molecular weight

42.966kDa

Background of BCAT1

BCAT1, Branched-chain Aminotransferase1, is also know as BCT1. The BCAT1 gene is highly expressed early in embryogenesis, and during organogenesis its expression is localized to the neural tube, the somites, and the mesonephric tubules. The gene is also expressed in several MYC-based tumors. The BCAT1 gene is mapped to chromosome 12. Lack of the enzyme BCT can cause auxotroph, a kind of auxotrophic mutant in Chinese-hamster ovary cells that lacks the ability to grow if alpha-ketoisovaleric acid, alpha-ketoisocaproic acid, and alpha-keto-beta-methylvaleric acid are substituted for valine, leucine, and isoleucine in the culture medium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For BCAT1 (Source: Uniprot.org, NCBI)

Gene Name

BCAT1

Full Name

Branched-chain-amino-acid aminotransferase, cytosolic

Weight

42.966kDa

Superfamily

class-IV pyridoxal-phosphate-dependent aminotransferase family

Alternative Names

BCAT(c); BCAT1; BCAT-1; BCATC; BCT1; BCT1PNAS121; branched chain amino-acid transaminase 1, cytosolic; branched chain aminotransferase 1, cytosolic; branched-chain-amino-acid aminotransferase, cytosolic; DKFZp686E12175; EC 2.6.1.42; ECA39; MECA39; placental protein 18; PNAS121; PP18; Protein ECA39 BCAT1 BCATC, BCT1, ECA39, MECA39, PNAS121, PP18 branched chain amino acid transaminase 1 branched-chain-amino-acid aminotransferase, cytosolic|branched chain amino-acid transaminase 1, cytosolic|branched chain aminotransferase 1, cytosolic|placental protein 18

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BCAT1, check out the BCAT1 Infographic

BCAT1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BCAT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-BCAT1 Antibody

Question

We are currently using anti-BCAT1 antibody PA1538 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

The anti-BCAT1 antibody (PA1538) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-09-17

Order DetailsPrice
PA1538

100μg

$370
PA1538-10ug

10μg sample (liquid)

$99
PA1538-Biotin

100 μg Biotin conjugated

$570
PA1538-Cy3

100 μg Cy3 conjugated

$570
PA1538-Dylight488

100 μg Dylight488 conjugated

$570
PA1538-Dylight550

100 μg Dylight550 conjugated

$570
PA1538-Dylight594

100 μg Dylight594 conjugated

$570
PA1538-FITC

100 μg FITC conjugated

$570
PA1538-HRP

100 μg HRP conjugated

$570
PA1538-APC

100 μg APC conjugated

$670
PA1538-PE

100 μg PE conjugated

$670
PA1538-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1538
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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