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Product Info Summary
| SKU: | A09611 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-CD85g LILRA4 Antibody
SKU/Catalog Number
A09611
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD85g LILRA4 Antibody catalog # A09611. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD85g LILRA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09611)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human LILRA4. AA range:391-440
Reactive Species
A09611 is reactive to LILRA4 in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
55.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09611 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC: 1:100-300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of MCF7 cells using CD85g Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of lysate from MCF7 cells, using LILRA4 Antibody.
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Immunohistochemical analysis of paraffin-embedded human-liver, antibody was diluted at 1:100
Specific Publications For Anti-CD85g LILRA4 Antibody (A09611)
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Customer Q&As
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6 Customer Q&As for Anti-CD85g LILRA4 Antibody
Question
Would anti-CD85g antibody A09611 work on monkey IHC with pituitary adenoma?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Our lab technicians have not tested anti-CD85g antibody A09611 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CD85g antibody A09611 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey pituitary adenoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cingulate cortex using anti-CD85g antibody A09611. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
Would A09611 anti-CD85g antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
You can see on the product datasheet, A09611 anti-CD85g antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-12-04
Question
you antibody to test anti-CD85g antibody A09611 on human cingulate cortex for research purposes, then I may be interested in using anti-CD85g antibody A09611 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Gonzalez
Verified customer
Asked: 2019-07-25
Answer
The products we sell, including anti-CD85g antibody A09611, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-25
Question
We are currently using anti-CD85g antibody A09611 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
S. Parker
Verified customer
Asked: 2018-02-14
Answer
The anti-CD85g antibody (A09611) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-14
Question
I have a question about product A09611, anti-CD85g antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
O. Walker
Verified customer
Asked: 2017-08-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09611 anti-CD85g antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-29
