Data & Images
|Product Name||Anti-CD89 Antibody|
|Description||Rabbit IgG polyclonal antibody for Immunoglobulin alpha Fc receptor(FCAR) detection. Tested with WB in Human.|
|Cite This Product||Anti-CD89 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1549)|
|Replacement Item||This antibody may replace the following items: sc-6261|sc-81633|sc-49197|sc-53669|sc-49195 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle of human CD89(84-101aa EFVIDHMDANKAGRYQCQ).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Immunoglobulin alpha Fc receptor(IgA Fc receptor)|
|Molecular Weight||32265 MW|
|Protein Function||Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production. .|
|Tissue Specificity||Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces. .|
|Subcellular Localization||Isoform A.1: Cell membrane; Single-pass type I membrane protein.|
|Alternative Names||Immunoglobulin alpha Fc receptor;IgA Fc receptor;CD89;FCAR;CD89;|
|Research Areas|||immunology|cell type markers|non-lineage||
Background for Immunoglobulin alpha Fc receptor(IgA Fc receptor)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-CD89 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-CD89 Antibody Images
Click the images to enlarge.
Lane 1: A549 Cell Lysate
Lane 2: U87 Cell Lysate
Lane 3: RAJI Cell Lysate
Lane 4: JURKAT Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,