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Product Info Summary
| SKU: | M05043 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CDCA5/Sororin Rabbit Monoclonal Antibody
SKU/Catalog Number
M05043
BM4790 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CDCA5/Sororin Rabbit Monoclonal Antibody catalog # M05043. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDCA5/Sororin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05043)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IAH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CDCA5
Reactive Species
M05043 is reactive to CDCA5 in Human
Observed Molecular Weight
35 kDa
Calculated molecular weight
27.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05043 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
FC 1:50
Positive Control
WB: human K562 whole cell, human U2OS whole cell
IHC: human colon tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Western blot analysis of CDCA5 using anti-CDCA5 antibody (M05043).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human U2OS whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDCA5 antigen affinity purified monoclonal antibody (Catalog # M05043) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDCA5 at approximately 35 kDa. The expected band size for CDCA5 is at 28 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon, using CDCA5 Antibody.
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Immunofluorescent analysis of Hela cells, using CDCA5 Antibody.
Specific Publications For Anti-CDCA5/Sororin Rabbit Monoclonal Antibody (M05043)
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Customer Q&As
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5 Customer Q&As for Anti-CDCA5/Sororin Rabbit Monoclonal Antibody
Question
We are currently using anti-CDCA5/Sororin Rabbit Monoclonal antibody M05043 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The anti-CDCA5/Sororin Rabbit Monoclonal antibody (M05043) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-22
Question
Will anti-CDCA5/Sororin Rabbit Monoclonal antibody M05043 work for IF with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
According to the expression profile of leukemic t-cell, CDCA5 is highly expressed in leukemic t-cell. So, it is likely that anti-CDCA5/Sororin Rabbit Monoclonal antibody M05043 will work for IF with leukemic t-cell.
Boster Scientific Support
Answered: 2019-10-24
Question
Is there a BSA free version of anti-CDCA5/Sororin Rabbit Monoclonal antibody M05043 available?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CDCA5/Sororin Rabbit Monoclonal antibody M05043 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-08-13
Question
Is this M05043 anti-CDCA5/Sororin Rabbit Monoclonal antibody reactive to the isotypes of CDCA5?
L. Jha
Verified customer
Asked: 2018-08-03
Answer
The immunogen of M05043 anti-CDCA5/Sororin Rabbit Monoclonal antibody is A synthesized peptide derived from human CDCA5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-08-03
Question
I see that the anti-CDCA5/Sororin Rabbit Monoclonal antibody M05043 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-03-13
