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- Table of Contents
CDK2 antibody (M00166-4) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IHC analysis of Cdk2 using anti-Cdk2 antibody (M00166-4).
Cdk2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
Cdk2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml mouse anti-Cdk2 Antibody (M00166-4) overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit (Catalog # SV0001) with DAB as the chromogen.
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Anti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5)
CDK2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | IHC, WB |
Tested Applications
CDK2 antibody was positively detected by WB in:
human Jurkat whole cell, human HepG2 whole cell, human U20S whole cell, rat heart tissue, rat L6 whole cell, mouse heart tissue, mouse C2C12 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CDK2 antibody was positively detected by IHC in:
human laryngeal squamous cell carcinomas tissue, human liver cancer tissue, human renal clear cell carcinoma tissue, human serous adenocarcinoma of ovary tissue, mouse colon tissue, rat colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5)
View all CDK2 Antibodies
View all CDK2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
6D5B5
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Cdk2 recombinant protein (Position: E81-L298). Human Cdk2 shares 98.6% amino acid (aa) sequence identity with rat Cdk2.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
30 kDa
Calculated Molecular Weight
33.9 kDa
Background of CDK2
CDK2, Cyclin-Dependent Kinase2, is also known as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65% sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.
Cite This Product
Anti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00166-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
100%
High Homology
Rat
99%
High Homology
Rabbit
99%
High Homology
Cow
99%
High Homology
Sheep
99%
High Homology
Zebrafish
89%
Moderate Homology
Drosophila
63%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CDK2 antibody
Immunohistochemistry (IHC) protocol for CDK2 antibody
Publications
Application:
Species:
Grape Seed Proanthocyanidin Extract Prevents Ovarian Aging by Inhibiting Oxidative Stress in the Hens
Published: 2018-01-09
Journal: Oxidative Medicine and Cellular Longevity
Application: WB
Impact Factor: 4.94
Species: Chicken
PMID: 29541349
Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro
Published: 2016-06-13
Journal: ACTA PHARMACOLOGICA SINICA
Application: WB
Impact Factor: 3.17
Species: Human
PMID: 27292614
Growth Inhibition Effects of Isoalantolactone on K562/A02 Cells: Caspase-dependent Apoptotic Pathways, S Phase Arrest, and Downregulation of Bcr/Abl
Published: 2014-05-27
Journal: PHYTOTHERAPY RESEARCH
Application: WB
Impact Factor: 2.40
Species: Human
PMID: 24865355
XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line
Published: 2009-10-31
Journal: CHEMICO-BIOLOGICAL INTERACTIONS
Application: WB
Impact Factor: 3.08
Species: Human
PMID: 19883635
Suppression of C-myc Expression Associates with Anti-Proliferation of Aloe-Emodin on Gastric Cancer Cells
Published: 2009-06-11
Journal: CANCER INVESTIGATION
Application: WB
Impact Factor: 1.98
Species: Human
PMID: 18443957
Chemically Modified Heparin Inhibits Mesangial Cell Proliferation Induced by High Glucose through Interfering with the Cell Cycle
Published: 2007-12-01
Journal: BIOLOGICAL & PHARMACEUTICAL BULLETIN
Application: WB
Impact Factor: 1.52
Species: Human
PMID: 18057711
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Product Promise
We promise Anti-Cdk2 Antibody Picoband® (monoclonal, 6D5B5) performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00166-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026