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- Table of Contents
CLDN5 antibody (RP1043) performs well in WB and shows reactivity with Human, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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Anti-Claudin 5 Picoband antibody, RP1043, Western blotting
All lanes: Anti Claudin-5 (RP1043) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 23KD
Observed bind size: 23KD
All lanes: Anti Claudin-5 (RP1043) at 0.5ug/ml
WB: Rat Liver Tissue Lysate at 50ug
Predicted bind size: 23KD
Observed bind size: 23KD
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Anti-Claudin 5/CLDN5 Antibody Picoband®
CLDN5 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Rat | Rabbit | WB |
Tested Applications
CLDN5 antibody was positively detected by WB in:
Rat Liver Tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Rat, Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Human
Predicted
Monkey
Predicted
Dog
Predicted
Bovine
Predicted
Sheep
Predicted
Product Information
Product Name
Anti-Claudin 5/CLDN5 Antibody Picoband®
View all CLDN5 Antibodies
View all CLDN5 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Claudin 5, different from the related mouse sequence by three amino acids and from the related rat sequence by four amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated Molecular Weight
23.1 kDa
Background of CLDN5
Claudin-5 (CLDN5) also known as BEC1, is a protein that in humans is encoded by the CLDN5 gene. By use of yeast artificial chromosomes, Claudin-5 is localized between polymorphic markers D22S944 and D22S941 on chromosome 22q11, both of which are deleted in more than 80% of VCFS patients. This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. What's more, it plays a major role in tight junction-specific obliteration of the intercellular space.
Cite This Product
Anti-Claudin 5/CLDN5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1043)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
96%
High Homology
Cow
93%
High Homology
Sheep
93%
High Homology
Mouse
89%
Moderate Homology
Rat
86%
Moderate Homology
Zebrafish
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CLDN5 antibody
Publications
Application:
Species:
HMGB1 promoted P-glycoprotein at the blood-brain barrier in MCAO rats via TLR4/NF-κB signaling pathway
Published: 2020-05-15
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC,WB
Impact Factor: 3.26
Species: Rat
PMID: 32417325
Brain Infection by Hepatitis E Virus Probably via Damage of the Blood-Brain Barrier Due to Alterations of Tight Junction Proteins
Published: 2019-03-19
Journal: Frontiers in Cellular and Infection Microbiology
Application: WB
Impact Factor: 3.52
Species: Human
PMID: 30949453
Effects of colistin on amino acid neurotransmitters and blood-brain barrier in the mouse brain
Published: 2016-03-25
Journal: NEUROTOXICOLOGY AND TERATOLOGY
Application: WB
Impact Factor: 2.49
Species: Mouse
PMID: 27018023
In vitro and in vivo study of dolichyl phosphate on the efflux activity of P-glycoprotein at the blood–brain barrier
Published: 2013-10-22
Journal: INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
Application: WB
Impact Factor: 2.69
Species: Rat
PMID: 24161469
Alteration in P-glycoprotein at the blood–brain barrier in the early period of MCAO in rats
Published: 2013-02-26
Journal: JOURNAL OF PHARMACY AND PHARMACOLOGY
Application: WB
Impact Factor: 2.03
Species: Rat
PMID: 23600383
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Product Promise
We promise Anti-Claudin 5/CLDN5 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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RP1043
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 7, 2026