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Product Info Summary
| SKU: | PA1233 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat, Zebrafish |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cullin 4B/CUL4B Antibody Picoband®
SKU/Catalog Number
PA1233
BA3374-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cullin 4B/CUL4B Antibody catalog # PA1233. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cullin 4B/CUL4B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1233)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1233 is reactive to CUL4B in Human, Mouse, Rat, Zebrafish
Observed Molecular Weight
104 kDa
Calculated molecular weight
104.0 kDa
Background of CUL4B
Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING (really interesting new gene) ubiquitin ligase (E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain (cullin homology domain) and a series of N-terminal repeats (cullin repeats). Ohtake et al. (2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor (AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B (AhR). Complex assembly and ubiquitin ligase activity of CUL4B (AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B (AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1233 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Zebrafish
Western blot, 0.1-0.5μg/ml, Human, Rat, Zebrafish, Mouse
Positive Control
WB: human Hela whole cell, human A549 whole cell, rat thymus tissue, rat kidney tissue, mouse kidney tissue
IHC: Zebrafish Body Tissue
Validation Images & Assay Conditions
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Anti-Cullin 4B antibody, PA1233, IHC(P)
IHC(P): Zebrafish Body Tissue
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Western blot analysis of Cullin 4B using anti-Cullin 4B antibody (PA1233).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human A549 whole cell lysate,
Lane 3: rat thymus tissue lysate,
Lane 4: rat kidney tissue lysate,
Lane 5: mouse kidney tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 4B antigen affinity purified polyclonal antibody (Catalog # PA1233) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 4B at approximately 104KD. The expected band size for Cullin 4B is at 104KD.
Specific Publications For Anti-Cullin 4B/CUL4B Antibody Picoband® (PA1233)
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3 Customer Q&As for Anti-Cullin 4B/CUL4B Antibody Picoband®
Question
Is this PA1233 anti-Cullin 4B/CUL4B antibody reactive to the isotypes of CUL4B?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The immunogen of PA1233 anti-Cullin 4B/CUL4B antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B(895-913aa DRDYMERDKENPNQYNYIA), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-27
Question
We are currently using anti-Cullin 4B/CUL4B antibody PA1233 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat, zebrafish. Is it true that the antibody can work on goat tissues as well?
J. Huang
Verified customer
Asked: 2019-04-29
Answer
The anti-Cullin 4B/CUL4B antibody (PA1233) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-29
Question
Is there a BSA free version of anti-Cullin 4B/CUL4B antibody PA1233 available?
D. Krishna
Verified customer
Asked: 2013-11-28
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Cullin 4B/CUL4B antibody PA1233 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-11-28
