Anti-Cullin 4B/CUL4B Antibody
Rabbit IgG polyclonal antibody for Cullin-4B(CUL4B) detection. Tested with WB, IHC-P in Human;Mouse;Rat;Zebrafish.
Anti-Cullin 4B/CUL4B Antibody Info At A Glance
|Reactivity:||Human, Mouse, Rat, Zebrafish|
|Product Name||Anti-Cullin 4B/CUL4B Antibody
See all CUL4B primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for CULLIN 4B/CUL4B detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. CULLIN 4B/CUL4B information: Molecular Weight: 103982 MW; Subcellular Localization: Nucleus .|
|Cite This Product||Anti-Cullin 4B/CUL4B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1233)|
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|Anti-Cullin 4B/CUL4B Antibody may replace the following items: sc 377462|sc 244056|sc 244055|sc 377410|sc 244057|sc 514422.|
|Reactivity/Species||Human, Mouse, Rat, Zebrafish|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Cullin 4B(895-913aa DRDYMERDKENPNQYNYIA), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For CUL4B (Source: Uniprot.org, NCBI)
|NCBI Gene Id||8450|
|Species Of This Entry||Human|
|Alternative Names||CUL4B|CUL-4B; cullin 4B; cullin-4B; DKFZp686F1470; KIAA0695; MRXHF2; MRXS15; MRXSC; SFM2|
|Post Tranlational Modifications||Ubiquitination; Phosphorylation; Methylation|
|Gene Location||Xq24, on Chromasome X, gene sequence: NC_000023.11 (120523858..120575829, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CUL4B (Source: Uniprot.org, NCBI)
|Protein Function||Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation- induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.|
|Interacting Proteins||CAND1; CDC37; DDB1; RBX1; UBXN7|
|Research Areas||Cell Cycle and Replication|
|Related Diseases||Malignant Neoplasms; Neoplasms; Dwarfism; Macrocephaly; Xeroderma Pigmentosum, Group E; Delayed Puberty; Xeroderma; Genomic Instability; Talipes Cavus; Xeroderma Pigmentosum; Physiological Stress; Intention Tremor; Hypogonadism; Cell Transformation, Neoplastic; Developmental Delay (disorder); Malnutrition|
|Related Pathways||Cell Cycle; Proteolysis; Dna Replication; S Phase; Cell Proliferation; Cell Growth; Pathogenesis; Localization; Cell Cycle Arrest; Tissue Development; Cognition; Mitosis; Conjugation; Rna Interference; Spermatogenesis; Cell Division; System Development; Stem Cell Proliferation; Dna Damage Checkpoint; Prophase|
|Background||Cullin 4B/CUL4B encodes a scaffold protein that organizes a cullin-RING(really interesting new gene) ubiquitin ligase(E3) complex in ubiquitylation. The CUL4 gene encodes a protein of 913 amino acids. The cullin domain is located between amino acid residues 217 and 815 and is characterized a by C-terminal globular domain(cullin homology domain) and a series of N-terminal repeats(cullin repeats). Ohtake et al.(2007) characterize a fat-soluble ligand-dependent ubiquitin ligase complex in human cell lines, in which dioxin receptor(AhR) is integrated as a component of a novel cullin 4B ubiquitin ligase complex, CUL4B(AhR). Complex assembly and ubiquitin ligase activity of CUL4B(AhR) in vitro and in vivo are dependent on the AhR ligand. In the CUL4B(AhR) complex, ligand-activated AhR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Their findings uncover a function for AhR as an atypical component of the ubiquitin ligase complex and demonstrate a non-genomic signalling pathway in which fat-soluble ligands regulate target-protein-selective degradation through a ubiquitin ligase complex.|
|Scientific References||PMID: 14578910 by Higa L.A., et al. Radiation-mediated proteolysis of CDT1 by CUL4-ROC1 and CSN complexes constitutes a new checkpoint.|
PMID: 8681378 by Kipreos E.T., et al. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family.
Our Boster Quality Guarantee for Anti-Cullin 4B/CUL4B Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Zebrafish, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Zebrafish, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
IHC(P): Zebrafish Body Tissue
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,
Lane 2: human A549 whole cell lysate,
Lane 3: rat thymus tissue lysate,
Lane 4: rat kidney tissue lysate,
Lane 5: mouse kidney tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cullin 4B antigen affinity purified polyclonal antibody (Catalog # PA1233) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cullin 4B at approximately 104KD. The expected band size for Cullin 4B is at 104KD.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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