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CXCL12 antibody (A00053-2) performs well in ELISA, IF, IHC and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 21 Publications

a00053 2 cxcl12 primary antibodies if testing 7

IF analysis of CXCL12 using anti-CXCL12 antibody (A00053-2).
CXCL12 was detected in a paraffin-embedded section of human hepatitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CXCL12 Antibody (A00053-2) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

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Anti-CXCL12 Antibody

CXCL12 antibody

21 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	ELISA, IF, IHC

Tested Applications

CXCL12 antibody was positively detected by IHC in:

human endometrial carcinoma tissue, rat spleen tissue, human tonsil tissue, human liver cancer tissue, human lymphoma tissue, mouse spleen tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

CXCL12 antibody was positively detected by IF in:

human hepatitis tissue, human tonsil tissue, mouse spleen tissue, rat spleen tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

ELISA

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Recommend

Sheep

Predicted

Monkey

Predicted

Dog

Predicted

Bovine

Predicted

Product Information

Product Name

Anti-CXCL12 Antibody
View all CXCL12 Antibodies

Form

Lyophilized

Uniprot

P48061

Gene ID

6387

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CXCL12 recombinant protein (Position: V24-M93).

Cross-Reactivity

No cross-reactivity with other proteins.

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Calculated Molecular Weight

10.7 kDa

Background of CXCL12

The stromal cell-derived factor 1 (SDF1), also known as C-X-C motif chemokine 12 (CXCL12), is a chemokine protein that in humans is encoded by the CXCL12 gene on chromosome 10. This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene.

Cite This Product

Anti-CXCL12 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00053-2)

Protocols

Immunohistochemistry (IHC) protocol for CXCL12 antibody

Review Protocol

Immunofluorescence (IF) protocol for CXCL12 antibody

Review Protocol

Publications

Collagen content and C-X-C motif chemokine ligand 12 expression in neoplastic breast stroma

Published: 2023-09-18

Journal: Revista da Associacao Medica Brasileira

Application: IHC

Impact Factor: 1.40

Species: Human

PMID: 37729354

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Free Secondary Antibody

Anti-CXCL12 Antibody

CXCL12 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Collagen content and C-X-C motif chemokine ligand 12 expression in neoplastic breast stroma

The CXCR4-AT1 axis plays a vital role in glomerular injury via mediating the crosstalk between podocyte and mesangial cell

Angelica sinensis polysaccharides prevents hematopoietic regression in D-Galactose-Induced aging model via attenuation of oxidative stress in hematopoietic microenvironment

Effect of isofebrifugine on the proliferation and invasion of human gastric cancer cells via MMP

MGF E peptide improves anterior cruciate ligament repair by inhibiting hypoxia-induced cell apoptosis and accelerating angiogenesis

Angelica sinensis Polysaccharides Ameliorate Stress-Induced Premature Senescence of Hematopoietic Cell via Protecting Bone Marrow Stromal Cells from Oxidative Injuries Caused by 5-Fluorouracil

Matrix elasticity-modified scaffold loaded with SDF-1α improves the in situ regeneration of segmental bone defect in rabbit radius.

Role of SDF-1/CXCR4 and cytokines in the development of ovary injury in chemotherapy drug induced premature ovarian failure mice

Treatment of radiation‑induced acute intestinal injury with bone marrow‑derived mesenchymal stem cells

Effect of Ermiao Fang with Xixin (Herba Asari Mandshurici) on bone marrow stem cell directional homing to a focal zone in an osteoarthritis rat model

Ultrasound-targeted stromal cell-derived factor-1-loaded microbubble destruction promotes mesenchymal stem cell homing to kidneys in diabetic nephropathy rats

Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF‑1α expression in vivo

Mesenchymal Stem Cell Transplantation Enhancement in Myocardial Infarction Rat Model under Ultrasound Combined with Nitric Oxide Microbubbles

CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma

Modulating the interaction of CXCR4 and CXCL12 by low-molecular-weight heparin inhibits hepatic metastasis of colon cancer

SDF-1/CXCR4-Mediated Migration of Transplanted Bone Marrow Stromal Cells Toward Areas of Heart Myocardial Infarction Through Activation of PI3K/Akt

Interaction of SDF-1α and CXCR4 plays an important role in pulmonary cellular infiltration in differentiation syndrome

Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer

Estrogen-replacement therapy promotes angiogenesis after acute myocardial infarction by enhancing SDF-1 and estrogen receptor expression

SDF-1α/CXCR4-mediated migration of systemically transplanted bone marrow stromal cells towards ischemic brain lesion in a rat model

Selective tropism of liver stem cells to hepatocellular carcinoma in vivo

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