Anti-CXCL12 Antibody Picoband™

Boster Bio Anti-CXCL12 Antibody Picoband™ catalog # A00053-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 13 publication(s).

Product Info Summary

SKU: A00053-2
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IHC, WB

Product Name

Anti-CXCL12 Antibody Picoband™

See all CXCL12 products

SKU/Catalog Number

A00053-2

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CXCL12 Antibody Picoband™ catalog # A00053-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CXCL12 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00053-2)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E. coli-derived human CXCL12 recombinant protein (Position: V24-M93).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins.

Reactive Species

A00053-2 is reactive to CXCL12 in Human, Mouse, Rat

Applications

A00053-2 is guaranteed for ELISA, IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Direct ELISA, 0.1-0.5μg/ml

Validation Images & Assay Conditions

Gene/Protein Information For CXCL12 (Source: Uniprot.Org, NCBI)

Uniprot ID

P48061

Gene ID

6387

Gene Name

CXCL12

Full Name

Stromal cell-derived factor 1

Weight

10.666kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1 CXCL12 IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1 C-X-C motif chemokine ligand 12 stromal cell-derived factor 1|chemokine (C-X-C motif) ligand 12|intercrine reduced in hepatomas|pre-B cell growth-stimulating factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCL12, check out the CXCL12 Infographic

CXCL12 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCL12: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00053-2 has been cited in 13 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

He K,Liu S,Xia Y,Xu J,Liu F,Xiao J,Li Y,Ding Q,Lu L,Xiang G,Zhan M.CXCL12 and IL7R as Novel Therapeutic Targets for Liver Hepatocellular Carcinoma Are Correlated With Somatic Mutations and the Tumor Immunological Microenvironment.Front Oncol.2020 Dec 4;10
Species: Human
A00053-2 usage in article: APP:IHC, SAMPLE:NP TISSUE, DILUTION:1:200

Matrix elasticity-modified scaffold loaded with SDF-1%u03B1 improves the in situ regeneration of segmental bone defect in rabbit radius

Effect of Ermiao Recipe (%u4E8C%u5999%u65B9) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in %u2026

Angelica sinensis Polysaccharides Ameliorate Stress-Induced Premature Senescence of Hematopoietic Cell via Protecting Bone Marrow Stromal Cells from Oxidative %u2026

iTRAQ-Based Quantitative Proteomic Comparison of Early- and Late-Passage Human Dermal Papilla Cell Secretome in Relation to Inducing Hair Follicle Regeneration

Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer

Treatment of radiation-induced acute intestinal injury with bone marrow-derived mesenchymal stem cells

Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF-1? expression?in vivo

CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma

Zhang W, Wang X, Yue K, Liu S, Liu X. Biomed Res Int. 2015;2015:195828. Doi: 10.1155/2015/195828. Epub 2015 Mar 19. Construction Of A Cxcl12-Kdel Fusion Gene To Inhibit Head And Neck Squamous Cell Carcinoma Metastasis By Intracellular Sequestratio...

Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.

Wu S, Li L, Wang G, Shen W, Xu Y, Liu Z, Zhuo Z, Xia H, Gao Y, Tan K. Int J Nanomedicine. 2014 Dec 3;9:5639-51. Doi: 10.2147/Ijn.S73950. Ecollection 2014. Ultrasound-Targeted Stromal Cell-Derived Factor-1-Loaded Microbubble Destruction Promotes Me...

Tong J, Ding J, Shen X, Chen L, Bian Y, Ma G, Yao Y, Yang F. Plos One. 2013 Nov 14;8(11):E80186. Doi: 10.1371/Journal.Pone.0080186. Ecollection 2013. Mesenchymal Stem Cell Transplantation Enhancement In Myocardial Infarction Rat Model Under Ultras...

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16 Customer Q&As for Anti-CXCL12 Antibody Picoband™

Question

Here is the WB image, lot number and protocol we used for liver using anti-CXCL12 antibody A00053-2. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-24

Question

We have seen staining in mouse liver. Any tips? Is anti-CXCL12 antibody supposed to stain liver positively?

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

Based on literature liver does express CXCL12. Based on Uniprot.org, CXCL12 is expressed in myometrium, fetal brain, fetal heart heart, fibroblast, liver, thymus uterus, brain, among other tissues. Regarding which tissues have CXCL12 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fetal brain, Fetal heart, and Heart, Pubmed ID: 16626895
Thymus, and Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-04-06

Question

Our lab used your anti-CXCL12 antibody for IHC on myometrium in a previous project. I am using rat, and I plan to use the antibody for ELISA next. Our lab want to know about examining myometrium as well as fetal brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?

Verified Customer

Verified customer

Asked: 2020-02-12

Answer

I looked at the website and datasheets of our anti-CXCL12 antibody and it seems that A00053-2 has been validated on rat in both IHC and ELISA. Thus A00053-2 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-02-12

Question

Is this A00053-2 anti-CXCL12 antibody reactive to the isotypes of CXCL12?

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

The immunogen of A00053-2 anti-CXCL12 antibody is E. coli-derived human CXCL12 recombinant protein (Position: V24-M93). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-10

Question

Our team were satisfied with the WB result of your anti-CXCL12 antibody. However we have observed positive staining in fibroblast secreted. using this antibody. Is that expected? Could you tell me where is CXCL12 supposed to be expressed?

A. Gonzalez

Verified customer

Asked: 2019-10-02

Answer

From what I have seen in literature, fibroblast does express CXCL12. Generally CXCL12 expresses in secreted. Regarding which tissues have CXCL12 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fetal brain, Fetal heart, and Heart, Pubmed ID: 16626895
Thymus, and Uterus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2019-10-02

Question

I was wanting to use your anti-CXCL12 antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?

Verified Customer

Verified customer

Asked: 2019-06-24

Answer

You can see on the product datasheet, A00053-2 anti-CXCL12 antibody has been validated for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-24

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CXCL12 antibody A00053-2. Let me know if you need anything else.

N. Rodriguez

Verified customer

Asked: 2019-04-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-04-18

Question

Does anti-CXCL12 antibody A00053-2 work for WB with liver?

Verified Customer

Verified customer

Asked: 2018-01-01

Answer

According to the expression profile of liver, CXCL12 is highly expressed in liver. So, it is likely that anti-CXCL12 antibody A00053-2 will work for WB with liver.

Boster Scientific Support

Answered: 2018-01-01

Question

I was wanting to use to test anti-CXCL12 antibody A00053-2 on rat liver for research purposes, then I may be interested in using anti-CXCL12 antibody A00053-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2017-12-07

Answer

The products we sell, including anti-CXCL12 antibody A00053-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-12-07

Question

Is there a BSA free version of anti-CXCL12 antibody A00053-2 available?

R. Li

Verified customer

Asked: 2017-08-11

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CXCL12 antibody A00053-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-08-11

Question

I see that the anti-CXCL12 antibody A00053-2 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-08-04

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-08-04

Question

Will A00053-2 anti-CXCL12 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-07-03

Answer

You can see on the product datasheet, A00053-2 anti-CXCL12 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-07-03

Question

We are currently using anti-CXCL12 antibody A00053-2 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?

A. Walker

Verified customer

Asked: 2015-12-09

Answer

The anti-CXCL12 antibody (A00053-2) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-12-09

Question

My question regarding product A00053-2, anti-CXCL12 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

R. Williams

Verified customer

Asked: 2015-10-15

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00053-2 anti-CXCL12 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-10-15

Question

We need using your anti-CXCL12 antibody for positive regulation of cell migration studies. Has this antibody been tested with western blotting on mouse spleen tissue? We would like to see some validation images before ordering.

L. Collins

Verified customer

Asked: 2014-11-04

Answer

We appreciate your inquiry. This A00053-2 anti-CXCL12 antibody is tested on human tonsil tissue, placenta tissue, a431 whole cell lysate, endometrial carcinoma tissue, mouse spleen tissue. It is guaranteed to work for ELISA, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2014-11-04

Question

Is a blocking peptide available for product anti-CXCL12 antibody (A00053-2)?

H. Rodriguez

Verified customer

Asked: 2013-12-09

Answer

We do provide the blocking peptide for product anti-CXCL12 antibody (A00053-2). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2013-12-09

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A00053-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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$50 fee for conjugation. Antibody size is reduced to 50ug.

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