Product Info Summary
SKU: | M00053 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, ICC, WB |
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Product info
Product Name
Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00053
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody catalog # M00053. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00053)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
IFO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SDF1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.
Reactive Species
M00053 is reactive to CXCL12 in Human
Applications
M00053 is guaranteed for IF, ICC, WB Boster Guarantee
Observed Molecular Weight
82 kDa
Calculated molecular weight
10.666kDa
Background of CXCL12
Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns (4,5) P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3) . PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of SDF1 expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CXCL12 (Source: Uniprot.org, NCBI)
Gene Name
CXCL12
Full Name
Stromal cell-derived factor 1
Weight
10.666kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1 CXCL12 IRH, PBSF, SCYB12, SDF1, TLSF, TPAR1 C-X-C motif chemokine ligand 12 stromal cell-derived factor 1|chemokine (C-X-C motif) ligand 12|intercrine reduced in hepatomas|pre-B cell growth-stimulating factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL12, check out the CXCL12 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL12: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody (M00053)
Hello CJ!
M00053 has been cited in 8 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Matrix elasticity-modified scaffold loaded with SDF-1%u03B1 improves the in situ regeneration of segmental bone defect in rabbit radius
Effect of Ermiao Recipe (%u4E8C%u5999%u65B9) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in %u2026
Angelica sinensis Polysaccharides Ameliorate Stress-Induced Premature Senescence of Hematopoietic Cell via Protecting Bone Marrow Stromal Cells from Oxidative %u2026
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF-1? expression?in vivo
Zhang W, Wang X, Yue K, Liu S, Liu X. Biomed Res Int. 2015;2015:195828. Doi: 10.1155/2015/195828. Epub 2015 Mar 19. Construction Of A Cxcl12-Kdel Fusion Gene To Inhibit Head And Neck Squamous Cell Carcinoma Metastasis By Intracellular Sequestratio...
Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.
Wu S, Li L, Wang G, Shen W, Xu Y, Liu Z, Zhuo Z, Xia H, Gao Y, Tan K. Int J Nanomedicine. 2014 Dec 3;9:5639-51. Doi: 10.2147/Ijn.S73950. Ecollection 2014. Ultrasound-Targeted Stromal Cell-Derived Factor-1-Loaded Microbubble Destruction Promotes Me...
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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5 Customer Q&As for Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody
Question
We have seen staining in human brain. Any tips? Is anti-SDF1 Rabbit Monoclonal antibody supposed to stain brain positively?
D. Parker
Verified customer
Asked: 2019-06-28
Answer
According to literature brain does express CXCL12. According to Uniprot.org, CXCL12 is expressed in myometrium, fetal brain, fetal heart heart, fibroblast, liver, thymus uterus, brain, among other tissues. Regarding which tissues have CXCL12 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fetal brain, Fetal heart, and Heart, Pubmed ID: 16626895
Thymus, and Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-28
Question
My boss were well pleased with the WB result of your anti-SDF1 Rabbit Monoclonal antibody. However we have observed positive staining in brain secreted. using this antibody. Is that expected? Could you tell me where is CXCL12 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
From what I have seen in literature, brain does express CXCL12. Generally CXCL12 expresses in secreted. Regarding which tissues have CXCL12 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fetal brain, Fetal heart, and Heart, Pubmed ID: 16626895
Thymus, and Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-02-19
Question
We need using your anti-SDF1 Rabbit Monoclonal antibody for response to ultrasound studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
K. Bhatt
Verified customer
Asked: 2014-07-21
Answer
We appreciate your inquiry. This M00053 anti-SDF1 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-07-21
Question
We are currently using anti-SDF1 Rabbit Monoclonal antibody M00053 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
J. Wu
Verified customer
Asked: 2014-03-17
Answer
The anti-SDF1 Rabbit Monoclonal antibody (M00053) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-17
Question
We ordered your anti-SDF1 Rabbit Monoclonal antibody for WB on fetal heart heart in the past. I am using human, and We want to use the antibody for ICC next. I am interested in examining fetal heart heart as well as thymus uterus in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
H. Johnson
Verified customer
Asked: 2013-01-18
Answer
I looked at the website and datasheets of our anti-SDF1 Rabbit Monoclonal antibody and it appears that M00053 has been tested on human in both WB and ICC. Thus M00053 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-01-18