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Product Info Summary
| SKU: | M00053 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00053
BM4834 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody catalog # M00053. Tested in WB, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00053)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
IFO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human SDF1
Reactive Species
M00053 is reactive to CXCL12 in Human
Observed Molecular Weight
11 kDa
Calculated molecular weight
10.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00053 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
ICC/IF 1:50-1:200
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of SDF1 expression in Jurkat cell lysate.
Specific Publications For Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody (M00053)
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Customer Q&As
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5 Customer Q&As for Anti-SDF1 CXCL12 Rabbit Monoclonal Antibody
Question
We have seen staining in human brain. Any tips? Is anti-SDF1 Rabbit Monoclonal antibody supposed to stain brain positively?
D. Parker
Verified customer
Asked: 2019-06-28
Answer
According to literature brain does express CXCL12. According to Uniprot.org, CXCL12 is expressed in myometrium, fetal brain, fetal heart heart, fibroblast, liver, thymus uterus, brain, among other tissues. Regarding which tissues have CXCL12 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fetal brain, Fetal heart, and Heart, Pubmed ID: 16626895
Thymus, and Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-28
Question
My boss were well pleased with the WB result of your anti-SDF1 Rabbit Monoclonal antibody. However we have observed positive staining in brain secreted. using this antibody. Is that expected? Could you tell me where is CXCL12 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
From what I have seen in literature, brain does express CXCL12. Generally CXCL12 expresses in secreted. Regarding which tissues have CXCL12 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Fetal brain, Fetal heart, and Heart, Pubmed ID: 16626895
Thymus, and Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-02-19
Question
We need using your anti-SDF1 Rabbit Monoclonal antibody for response to ultrasound studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
K. Bhatt
Verified customer
Asked: 2014-07-21
Answer
We appreciate your inquiry. This M00053 anti-SDF1 Rabbit Monoclonal antibody is tested on jurkat cell lysate. It is guaranteed to work for IF, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-07-21
Question
We are currently using anti-SDF1 Rabbit Monoclonal antibody M00053 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
J. Wu
Verified customer
Asked: 2014-03-17
Answer
The anti-SDF1 Rabbit Monoclonal antibody (M00053) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-17
Question
We ordered your anti-SDF1 Rabbit Monoclonal antibody for WB on fetal heart heart in the past. I am using human, and We want to use the antibody for ICC next. I am interested in examining fetal heart heart as well as thymus uterus in our next experiment. Do you have any suggestion on which antibody would work the best for ICC?
H. Johnson
Verified customer
Asked: 2013-01-18
Answer
I looked at the website and datasheets of our anti-SDF1 Rabbit Monoclonal antibody and it appears that M00053 has been tested on human in both WB and ICC. Thus M00053 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-01-18
