This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CYP1A1 antibody (PB9544) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
Filter:
Flow Cytometry analysis of CACO-2 cells using anti-CYP1A1 antibody (PB9544).
Overlay histogram showing CACO-2 cells stained with PB9544 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (PB9544,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing CACO-2 cells stained with PB9544 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CYP1A1 Antibody (PB9544,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Designing an IHC experiment for CYP1A1? The guide covers the expected staining pattern, antigen retrieval, antibodies backed by real IHC images, tissue controls, and protocols taken from published papers.
Open the CYP1A1 IHC GuideBundle Save
Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband®
CYP1A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
CYP1A1 antibody was positively detected by WB in:
human Hela whole cell, rat liver tissue, rat RH-35 whole cell, rat PC-12 whole cell, mouse liver tissue, mouse HEPA1/6 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CYP1A1 antibody was positively detected by IHC in:
Mouse Kidney tissue, Rat Kidney tissue, Human Mammary Cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
CYP1A1 antibody was positively detected by IHC-F in:
Mouse Kidney tissue, Human Placenta tissue
CYP1A1 antibody was positively detected by ICC/IF in:
Caco-2 cell
CYP1A1 antibody was positively detected by FC in:
CACO-2 cell, Hela cell, K562 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human, Mouse, -
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Human
Tested
Tested
Recommend
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband®
View all CYP1A1 Antibodies
View all CYP1A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CYP1A1 recombinant protein (Position: H183-D320). Human CYP1A1 shares 81.2% amino acid (aa) sequence identity with both mouse and rat CYP1A1.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated Molecular Weight
58.2 kDa
Background of CYP1A1
CYP1A1 is involved in phase I xenobiotic and drug metabolism (one substrate of it is theophylline). It is inhibited by fluoroquinolones and macrolides and induced by aromatic hydrocarbons. CYP1A1 is also known as AHH (aryl hydrocarbon hydroxylase). It is involved in the metabolic activation of aromatic hydrocarbons (polycyclic aromatic hydrocarbons, PAH), for example, benzo (a)pyrene (BP), by transforming it to an epoxide. In this reaction, the oxidation of benzo[a]pyrene is catalysed by CYP1A1 to form BP-7,8-epoxide, which can be further oxidized by epoxide hydrolase (EH) to form BP-7,8-dihydrodiol. Finally CYP1A1 catalyses this intermediate to form BP-7,8-dihydrodiol-9,10-epoxide, which is the ultimate carcinogen. However, an in vivo experiment with gene-deficient mice has found that the hydroxylation of benzo (a)pyrene by CYP1A1 can have an overall protective effect on the DNA, rather than contributing to potentially carcinogenic DNA modifications. This effect is likely due to the fact that CYP1A1 is highly active in the intestinal mucosa, and thus inhibits infiltration of ingested benzo (a)pyrene carcinogen into the systemic circulation.
Cite This Product
Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9544)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
92%
High Homology
Mouse
81%
Moderate Homology
Rat
81%
Moderate Homology
Cow
80%
Moderate Homology
Sheep
80%
Moderate Homology
Dog
80%
Moderate Homology
Rabbit
75%
Moderate Homology
Zebrafish
62%
Low homology
Drosophila
30%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CYP1A1 antibody
Immunohistochemistry (IHC) protocol for CYP1A1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for CYP1A1 antibody
Flow Cytometry (FC) protocol for CYP1A1 antibody
Publications
Application:
Species:
Stabilization of MHCII on dendritic cells via AhR-mediated blockade of proteasomal degradation drives Th17 responses to exogenous antigens
Published: 2026-06-12
Journal: INTERNATIONAL IMMUNOPHARMACOLOGY
Application: WB
Impact Factor: 5.60
Species: Mouse,Human
PMID: 42284760
Niuhuang Jiedu Tablet's compatibility alleviates realgar-induced intestinal toxicity by activating aryl hydrocarbon receptor pathway via Akkermansia and indole−3−lactate
Published: 2025-07-07
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Mouse
PMID: 40633858
Iron-doped diesel exhaust early-in-life inhalation-induced cardiopulmonary toxicity in chicken embryo: Roles of ferroptosis and acyl hydrocarbon signaling
Published: 2024-10-05
Journal: ENVIRONMENTAL POLLUTION
Application: WB
Impact Factor: 7.60
Species: Chicken
PMID: 39374763
Kynurenine Attenuates Ulcerative Colitis Mediated by the Aryl Hydrocarbon Receptor
Published: 2024-09-13
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 5.70
Species: Mouse,Human
PMID: 39271472
Effect of polyaromatic hydrocarbons on cellular cytochrome P450 1A induction
Published: 2021-12-06
Journal: Ocean and Coastal Research
Application: WB
Impact Factor: 0.00
Species: Siganus rivulatus
Review
Have you used Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Cytochrome P450 1A1/CYP1A1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PB9544
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 27, 2026