DAP3 antibody (A05131-3) performs well in ELISA, IHC, WB and shows reactivity with Human, Mouse, Rat.

  • Validated for inter-lot consistency, specificity, and reproducibility
  • Committed to quality since 1993
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Anti-DAP3 Antibody Picoband®

DAP3 antibody

Reactive SpeciesHostApplication
Human, Mouse, RatRabbitELISA, IHC, WB

SKU: A05131-3

Size: 100 μg

Bundle to save: I do not want free secondary ab
Price:
$370/100 μg

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Quantity: 1

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Tested Applications

DAP3 antibody was positively detected by WB in:

human K562 whole cell, human THP-1 whole cell, human Hela whole cell, human HepG2 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

DAP3 antibody was positively detected by IHC in:

human colon cancer tissue

Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Recommend Dilution

Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
ELISA
0.1-0.5 μg/ml

* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.

Reactive Data

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WB
IHC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Mouse
Recommend
Rat
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted

Product Information

Product Name
Anti-DAP3 Antibody Picoband®
View all DAP3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human DAP3 recombinant protein (Position: M1-L398).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
40 kDa
Calculated Molecular Weight

45.6 kDa

Background of DAP3
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that also participates in apoptotic pathways which are initiated by tumor necrosis factor-alpha, Fas ligand, and gamma interferon. This protein potentially binds ATP/GTP and might be a functional partner of the mitoribosomal protein S27. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q.
Cite This Product
Anti-DAP3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05131-3)

Protocols

Western Blot protocol for DAP3 antibody
Immunohistochemistry (IHC) protocol for DAP3 antibody

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Product Promise

We promise Anti-DAP3 Antibody Picoband® performs excellently in relevant applications for ELISA, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more

In stock
Order Product
A05131-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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Guaranteed product quality

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Oct 25, 2026