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Product Info Summary
| SKU: | A05604-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DFNA5 Antibody
SKU/Catalog Number
A05604-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DFNA5 Antibody catalog # A05604-1. Tested in WB, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DFNA5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05604-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from DFNA5 . at AA range: 200-280
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A05604-1 is reactive to DFNA5 in Human, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
54.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05604-1 is guaranteed for ELISA, WB Boster Guarantee
Recommend Dilution
WB 1:500-1:2000
ELISA 1:40000
Validation Images & Assay Conditions
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Western blot analysis of extracts from rat stomach, 293 cells, using DFNA5 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Specific Publications For Anti-DFNA5 Antibody (A05604-1)
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Customer Q&As
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5 Customer Q&As for Anti-DFNA5 Antibody
Question
Would A05604-1 anti-DFNA5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
As indicated on the product datasheet, A05604-1 anti-DFNA5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-30
Question
Is this A05604-1 anti-DFNA5 antibody reactive to the isotypes of DFNA5?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
The immunogen of A05604-1 anti-DFNA5 antibody is Synthesized peptide derived from the Internal region of human DFNA5. at AA rangle: 200-280. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-07-26
Question
I see that the anti-DFNA5 antibody A05604-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-20
Question
Is a blocking peptide available for product anti-DFNA5 antibody (A05604-1)?
O. Johnson
Verified customer
Asked: 2016-02-03
Answer
We do provide the blocking peptide for product anti-DFNA5 antibody (A05604-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2016-02-03
Question
We are currently using anti-DFNA5 antibody A05604-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on goat tissues as well?
W. Li
Verified customer
Asked: 2013-05-22
Answer
The anti-DFNA5 antibody (A05604-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-05-22
