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Product Info Summary
| SKU: | A12099 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-DHHC-15 ZDHHC15 Antibody
SKU/Catalog Number
A12099
Size
100μl
Form
Liquid
Description
Boster Bio Anti-DHHC-15 ZDHHC15 Antibody catalog # A12099. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DHHC-15 ZDHHC15 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12099)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ZDHHC15. AA range:288-337
Reactive Species
A12099 is reactive to ZDHHC15 in Human, Mouse, Rat
Calculated molecular weight
39.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12099 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blot analysis of the lysates from 293 cells using ZDHHC15 antibody.
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Western Blot analysis of Jurkat cells using DHHC-15 Polyclonal Antibody
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Western blot analysis of lysates from Jurkat cells, using ZDHHC15 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-DHHC-15 ZDHHC15 Antibody (A12099)
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Customer Q&As
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4 Customer Q&As for Anti-DHHC-15 ZDHHC15 Antibody
Question
We are currently using anti-DHHC-15 antibody A12099 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-13
Answer
The anti-DHHC-15 antibody (A12099) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-13
Question
I have a question about product A12099, anti-DHHC-15 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Wu
Verified customer
Asked: 2020-02-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A12099 anti-DHHC-15 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-10
Question
Does anti-DHHC-15 antibody A12099 work on monkey WB with brain teratocarcinoma?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
Our lab technicians have not tested anti-DHHC-15 antibody A12099 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-DHHC-15 antibody A12099 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain teratocarcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-21
Question
Our lab want to know about to test anti-DHHC-15 antibody A12099 on human vagina for research purposes, then I may be interested in using anti-DHHC-15 antibody A12099 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Miller
Verified customer
Asked: 2019-04-09
Answer
The products we sell, including anti-DHHC-15 antibody A12099, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-04-09
