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Product Info Summary
| SKU: | M06819-23 |
|---|---|
| Size: | 100ug |
| Reactive Species: | Human, Vertebrates |
| Host: | Rabbit |
| Application: | ChIP, ELISA, IHC, WB, Luminex |
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Product info
Product Name
Anti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181
SKU/Catalog Number
M06819-23
Size
100ug
Form
Liquid
Description
Boster Bio Anti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181 (Catalog # M06819-23). Tested in WB, IHC, ChIP, ELISA, Luminex applications. This antibody reacts with Human, Vertebrates.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06819-23)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM181
Isotype
Rabbit IgG
Immunogen
A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys79).
Cross-reactivity
This antibody reacts to Histone H3 dimethylated at Lysine 79 (K79me2). No cross-reactivity with monomethylated Lysine 79(K79me1) or trimethylated Lysine 79 (K79me3), or other methylation in histone H3.
Reactive Species
M06819-23 is reactive to H3F3A in Human, Vertebrates
Calculated molecular weight
15.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06819-23 is guaranteed for ChIP, ELISA, IHC, WB, Luminex Boster Guarantee
Recommend Dilution
WB: 0.25 ug/mL – 1 ug/mL
IHC: 0.1 ug/mL – 1 ug/mL
ChIP: 2 ug/mL- 10 ug.mL
ELISA: 0.2 ug/mL - 1 ug/mL
Luminex: 0.1 ug/mL – 0.5 ug/mL.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 ug/mL, showed a band of histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
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Specificity Test result
RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.
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IHC result
Immunohistochemistry staining of HepG2 cell using anti-Dimethyl-Histone H3 (Lys79) antibody, RM181.
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ChIP result
ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5ug). Real-time PCR was performed using primers specific to the gene indicated.
Specific Publications For Anti-Dimethyl Histone H3 (Lys79) H3F3A Rabbit Monoclonal Antibody, Clone#RM181 (M06819-23)
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