Anti-Dopamine Receptor D3/DRD3 Antibody
Rabbit IgG polyclonal antibody for D(3) dopamine receptor(DRD3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-Dopamine Receptor D3/DRD3 Antibody
See all DRD3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for D3DR/DRD3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. D3DR/DRD3 information: Molecular Weight: 44225 MW; Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells; Tissue Specificity: Brain.|
|Cite This Product||Anti-Dopamine Receptor D3/DRD3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1584)|
|Specificity||Anti-Dopamine Receptor D3/DRD3 Antibody (PA1584) reacts with Human, Mouse, Rat DRD3, in native form and recombinant. Superfamily members of DRD3 are not reactive to PA1584.|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Dopamine Receptor D3(352-367aa NTHCQTCHVSPELYSA), different from the related rat and mouse sequences by two amino acids.|
|Reactivity||Human, Mouse, Rat|
Our Boster Quality Guarantee for Anti-Dopamine Receptor D3/DRD3 Antibody covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-Dopamine Receptor D3/DRD3 Antibody (PA1584).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-Dopamine Receptor D3 antibody, PA1584, IHC(P)
IHC(P): Rat Brain Tissue
Anti-Dopamine Receptor D3 antibody, PA1584, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: U87 Cell Lysate
Lane 4: HELA Cell Lysate
Protein Target Info (Source: Uniprot.org)
|Protein Name||D(3) dopamine receptor|
|Alternative Names||D(3) dopamine receptor;Dopamine D3 receptor;DRD3;|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells.|
|Molecular Weight||44225 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation. .|
|Research Areas||Human, Mouse, Rat
*You can search these to find other products in these research areas.
|Background||DRD3 is a dopamine receptor that differs in its pharmacology and signaling system from the D1 and D2 receptors and represents both an autoreceptor and a postsynaptic receptor. DRD3 is localized to limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions. The DRD3 gene, which is mapped to chromosome 3, is like the DRD2 gene but unlike most other members of this superfamily and it contains introns, 5 in number. The position of 2 of the introns corresponds to that of introns in DRD2. The D3 receptor appeared to mediate some of the effects of antipsychotic drugs and drugs used in the treatment of Parkinson disease, which were previously though to interact only with D2 receptors.|
Other Recommended Resources
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Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WBA: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.