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Product Info Summary
| SKU: | PA1584 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Dopamine Receptor D3/DRD3 Antibody Picoband®
SKU/Catalog Number
PA1584
BA0805 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Dopamine Receptor D3/DRD3 Antibody catalog # PA1584. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Dopamine Receptor D3/DRD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1584)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Dopamine Receptor D3, different from the related rat and mouse sequences by two amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1584 is reactive to DRD3 in Human, Mouse, Rat
Observed Molecular Weight
59 kDa
Calculated molecular weight
44.2 kDa
Background of DRD3
DRD3 is a dopamine receptor that differs in its pharmacology and signaling system from the D1 and D2 receptors and represents both an autoreceptor and a postsynaptic receptor. DRD3 is localized to limbic areas of the brain, which are associated with cognitive, emotional, and endocrine functions. The DRD3 gene, which is mapped to chromosome 3, is like the DRD2 gene but unlike most other members of this superfamily and it contains introns, 5 in number. The position of 2 of the introns corresponds to that of introns in DRD2. The D3 receptor appeared to mediate some of the effects of antipsychotic drugs and drugs used in the treatment of Parkinson disease, which were previously though to interact only with D2 receptors.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1584 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Positive Control
WB: human U87 whole cell, human SH-SY5Y whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell
IHC: rat brain tissue, human brain tissue
Validation Images & Assay Conditions
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Western blot analysis of DRD3 using anti-DRD3 antibody (PA1584).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human SH-SY5Y whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DRD3 antigen affinity purified polyclonal antibody (PA1584) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for DRD3 at approximately 59 kDa. The expected band size for DRD3 is at 44 kDa.
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IHC analysis of DRD3 using anti-DRD3 antibody (PA1584).
DRD3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRD3 Antibody (PA1584) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of DRD3 using anti-DRD3 antibody (PA1584).
DRD3 was detected in a paraffin-embedded section of human brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-DRD3 Antibody (PA1584) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Dopamine Receptor D3/DRD3 Antibody Picoband® (PA1584)
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1 Customer Q&As for Anti-Dopamine Receptor D3/DRD3 Antibody Picoband®
Question
We are currently using anti-Dopamine Receptor D3/DRD3 antibody PA1584 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
The anti-Dopamine Receptor D3/DRD3 antibody (PA1584) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-23
