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- Table of Contents
DYNLT1 antibody (A05556) performs well in ELISA, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of DYNLT1 using anti-DYNLT1 antibody (A05556).
DYNLT1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DYNLT1 Antibody (A05556) overnight at 4°C. Cy2 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
DYNLT1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-DYNLT1 Antibody (A05556) overnight at 4°C. Cy2 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-DYNLT1 Antibody Picoband®
DYNLT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IF, IHC, WB |
Tested Applications
DYNLT1 antibody was positively detected by WB in:
human SiHa whole cell, human U251 whole cell, human SH-SY5Y whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
DYNLT1 antibody was positively detected by IHC in:
human colon adenocarcinoma tissue, human esophageal keratinizing squamous cell carcinoma tissu, human testis cancer tissue, human lung cancer tissue, mouse epididymis tissue, mouse testis tissue, rat testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
DYNLT1 antibody was positively detected by IF in:
human placenta tissue, human testicular cancer tissue, rat testis tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunofluorescence
5μg/ml
Human, Rat
ELISA
0.1-0.5μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
ELISA
Human
Tested
Tested
Tested
Recommend
Mouse
Tested
Recommend
Recommend
Rat
Tested
Tested
Recommend
Sheep
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-DYNLT1 Antibody Picoband®
View all DYNLT1 Antibodies
View all DYNLT1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human DYNLT1 recombinant protein (Position: M1-A80).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated Molecular Weight
12.5 kDa
Background of DYNLT1
Dynein light chain Tctex-type 1 is a protein that in humans is encoded by the DYNLT1 gene. This gene encodes a component of the motor complex, cytoplasmic dynein, which transports cellular cargo along microtubules in the cell. The encoded protein regulates the length of primary cilia which are sensory organelles found on the surface of cells. The protein encoded by this gene interacts with viral proteins, like the minor capsid protein L2 of human papillomavirus, and is required for dynein-mediated delivery of the viral nucleic acid to the host nucleus. This protein interacts with oncogenic nucleoporins to disrupt gene regulation and cause leukemic transformation. Pseudogenes of this gene are present on chromosomes 4 and 17. Alternative splicing results in multiple transcript variants encoding different isoforms.
Cite This Product
Anti-DYNLT1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05556)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Rabbit
99%
High Homology
Rat
98%
High Homology
Mouse
93%
High Homology
Zebrafish
68%
Low homology
Drosophila
66%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for DYNLT1 antibody
Immunohistochemistry (IHC) protocol for DYNLT1 antibody
Immunofluorescence (IF) protocol for DYNLT1 antibody
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Product Promise
We promise Anti-DYNLT1 Antibody Picoband® performs excellently in relevant applications for ELISA, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A05556
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026