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Product Info Summary
| SKU: | M32743 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-ECRG4 Mouse Monoclonal Antibody [Clone ID: OTI3B10]
SKU/Catalog Number
M32743
Size
100 µl
Description
Boster Bio C2orf40 mouse monoclonal antibody, clone OTI3B10 (formerly 3B10). Catalog# M32743. Tested in FC, IF, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ECRG4 Mouse Monoclonal Antibody [Clone ID: OTI3B10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M32743)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3B10
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human C2orf40 (NP_115787) produced in HEK293T cell.
Reactive Species
M32743 is reactive to ECRG4 in Human
Calculated molecular weight
17.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M32743 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY C2orf40 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-C2orf40.
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Immunofluorescent staining of HeLa cells using anti-C2orf40 mouse monoclonal antibody (M32743).
Specific Publications For Anti-ECRG4 Mouse Monoclonal Antibody [Clone ID: OTI3B10] (M32743)
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