Anti-EDA Antibody

Rabbit Polyclonal EDA Antibody. Validated in WB and tested in Human, Mouse, Rat.

SKU PA1561
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-EDA Antibody
See all EDA primary antibodies, ELISA kits and proteins
SKU/Catalog Number PA1561
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit Polyclonal EDA Antibody. Validated in WB and tested in Human, Mouse, Rat. Size: 100μg/vial.
Cite This Product Anti-EDA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1561)
Specificity Anti-EDA Antibody (PA1561) reacts with Human, Mouse, Rat EDA, in native form and recombinant. Superfamily members of EDA are not reactive to PA1561.
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Form Lyophilized
Immunogen Anti-EDA Antibody (PA1561) was raised against A synthetic peptide corresponding to a sequence in the middle region of human EDA(254-269aa HLQGQGSAIQVKNDLS), identical to the related mouse and rat sequences..
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-EDA Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-EDA Antibody (PA1561).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/antibody/pa1561 1 WB anti eda eda a2 antibody.jpg

Anti-EDA antibody, PA1561, Western blotting
WB: SW620 Cell Lysate

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q92838
Gene Name EDA
Protein Name Ectodysplasin-A
Tissue Specificity Not abundant; expressed in specific cell types of ectodermal (but not mesodermal) origin of keratinocytes, hair follicles, sweat glands. Also in adult heart, liver, muscle, pancreas, prostate, fetal liver, uterus, small intestine and umbilical chord. .
Alternative Names Ectodysplasin-A;Ectodermal dysplasia protein;EDA protein;Ectodysplasin-A, membrane form;Ectodysplasin-A, secreted form;EDA;ED1, EDA2;Show more
Subcellular Localization Cell membrane; Single-pass type II membrane protein.
Molecular Weight 41294 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Seems to be involved in epithelial-mesenchymal signaling during morphogenesis of ectodermal organs. Isoform 1 binds only to the receptor EDAR, while isoform 3 binds exclusively to the receptor XEDAR.
Research Areas Developmental Biology, Growth Factors/Hormones, Lineage Markers, Lineage Specification, Signal Transduction, Stem Cells

*You can search these to find other products in these research areas.
Background Anhidrotic ectodermal dysplasia(EDA) is an X-linked recessive disorder which affects ectodermal structures. Ectodysplasin-A, the protein encoded by the EDA gene, is a member of the tumor necrosis factor ligand superfamily that forms a collagen triple helix, suggesting functions in signal transduction and cell adhesion. Wnt signaling does control EDA gene expression, but ectodysplasin-A does not feedback on the Wnt pathway.

Other Recommended Resources

Here are featured tools and databases that you might find useful.

Publishing with Anti-EDA Antibody (PA1561)? Please let us know so we can cite the reference in this product datasheet. Email us at support@bosterbio.com.

Order Product (PA1561)

Promotion:

Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
Option Price
30ug sample size $99
100ug $240
100ug+Free HRP Secondary BA1054 $240
100ug+Free Biotin Secondary BA1003 $240

USD $240

Ships next business day.

Troubleshooting

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Publications

X-irradiation for inhibiting glial scar formation in injured spinal cord

Customer Q&As

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.