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- Table of Contents
FABP2 antibody (PB9943) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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IHC analysis of FABP2/I-FABP using anti-FABP2/I-FABP antibody (PB9943).
FABP2/I-FABP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP2/I-FABP Antibody (PB9943) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
FABP2/I-FABP was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-FABP2/I-FABP Antibody (PB9943) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Anti-Intestinal FABP/FABP2 Antibody Picoband®
FABP2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
FABP2 antibody was positively detected by WB in:
SW620 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FABP2 antibody was positively detected by IHC in:
mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FABP2 antibody was positively detected by ICC/IF in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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IHC
IF
Mouse
Tested
Recommend
Rat
Tested
Recommend
Human
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-Intestinal FABP/FABP2 Antibody Picoband®
View all FABP2 Antibodies
View all FABP2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human FABP2/I-FABP, different from the related mouse sequence by seven amino acids, and from the related rat sequence by six amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
15 kDa
Calculated Molecular Weight
15.2 kDa
Background of FABP2
FABP 2, Fatty acid-binding protein 2, is a protein that in humans is encoded by the FABP2 gene. Using a human cDNA probe, the gene is assigned to chromosome 4 in somatic cell hybrids. FABP 2 gene contains four exons and is an abundant cytosolic protein in small intestine epithelial cells. The FABPs belong to a multigene family with nearly twenty identified members. And FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. They form 14-15 kDa proteins and are thought to participate in the uptake, intracellular metabolism and/or transport of long-chain fatty acids. Also, they may be responsible in the modulation of cell growth and proliferation.
Cite This Product
Anti-Intestinal FABP/FABP2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9943)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
92%
High Homology
Rabbit
89%
Moderate Homology
Cow
86%
Moderate Homology
Sheep
86%
Moderate Homology
Rat
84%
Moderate Homology
Dog
84%
Moderate Homology
Mouse
81%
Moderate Homology
Zebrafish
78%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FABP2 antibody
Immunohistochemistry (IHC) protocol for FABP2 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for FABP2 antibody
Publications
Application:
Species:
Dynamic regulation of N6,2′-O-dimethyladenosine (m6Am) in obesity
Published: 2021-12-10
Journal: Nature Communications
Application: WB
Impact Factor: 14.92
Species: Mouse
PMID: 34893620
Identification and expression analysis of fabp2 gene from common carp Cyprinus carpio
Published: 2012-02-02
Journal: JOURNAL OF FISH BIOLOGY
Application: WB,IHC
Impact Factor: 1.69
Species: Fish
PMID: 22380561
Review
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Product Promise
We promise Anti-Intestinal FABP/FABP2 Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PB9943
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026