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- Table of Contents

FTH1 antibody (M02401) performs well in IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, ICC, WB |
FTH1 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human K562 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, mouse HEPA1-6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
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| SKU | M02401 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampus tissue |
| Sample Processing Description | The tissue was minced and sonicated, then lysed on ice for 1 hour using RIPA buffer. After centrifugation to collect the supernatant and protein quantification by BCA, samples were mixed with loading buffer at the appropriate ratio and denatured by boiling in a water bath. Fifteen microliters of each protein sample were loaded per lane onto SDS-PAGE gel. |
| Primary Antibody | Anti-Ferritin FTH1 Rabbit Monoclonal Antibody |
| Primary Incubation | overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon |
| Results Summary | The FTH1 antibody was used to detect the expression of the target protein in human uterine tissue. The WB bands were single and clear, and compared with other domestic and international brands, this antibody offers excellent cost-performance. |
Anfeng Ning, Peking University Third Hospital
Verified customer
Submitted 2025-11-06
We promise Anti-Ferritin FTH1 Rabbit Monoclonal Antibody performs excellently in relevant applications for IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.