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Product Info Summary
| SKU: | PA1216 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Fibroblast growth factor 8 FGF8 Antibody Picoband®
SKU/Catalog Number
PA1216
BA0854 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fibroblast growth factor 8 FGF8 Antibody catalog # PA1216. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibroblast growth factor 8 FGF8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1216)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human FGF8, identical to the related rat and mouse sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1216 is reactive to FGF8 in Human, Mouse, Rat
Observed Molecular Weight
272 kDa
Calculated molecular weight
26.5 kDa
Background of FGF8
Fibroblast growth factor 8 (androgen-induced), also known as FGF8 or AIGF, is a human gene which maps to 10q24. The protein encoded by this gene are secreted proteins that interact with FGF tyrosine kinase receptors to mediate growth and development. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The temporal and spatial patterns of this gene expression suggest that FGF8 is involved in gastrulation, regionalization of the brain, and organogenesis of the limb and face as an embryonic epithelial factor. The adult expression of FGF8 is restricted to gonads, including testes and ovaries. FGF8 stimulated growth of human prostate carcinoma cells and mouse fibroblasts and mammary carcinoma cells in a dose-dependent manner. It also may play an important role in growth and patterning of limbs, face, and central nervous system. FGF8 is expressed in increased levels in breast cancer and in lactating human breast; it was also detected in human milk. A survey of other normal tissues showed that FGF8 is expressed in the proliferative cells of the skin and epithelial cells in colon, ovary, fallopian tube, and uterus. FGF8 in adults expression is restricted to the gonads. We detected rat ovary in our lab.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1216 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Ovary Tissue
IHC: Rat Ovary Tissue, human ovarian cancer tissue
Validation Images & Assay Conditions
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Anti-FGF8 antibody, PA1216, Western blotting
WB: Rat Ovary Tissue Lysate
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Anti-FGF8 antibody, PA1216, IHC(P)
IHC(P): Rat Ovary Tissue
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IHC analysis of FGF8 using anti-FGF8 antibody (PA1216).
FGF8 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-FGF8 Antibody (PA1216) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Fibroblast growth factor 8 FGF8 Antibody Picoband® (PA1216)
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4 Customer Q&As for Anti-Fibroblast growth factor 8 FGF8 Antibody Picoband®
Question
I was wanting to use your anti-FGF8 antibody for WB for rat esophageal carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat esophageal carcinoma identification?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
It shows on the product datasheet, PA1216 anti-FGF8 antibody has been validated for IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat esophageal carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
Is this PA1216 anti-FGF8 antibody reactive to the isotypes of FGF8?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
The immunogen of PA1216 anti-FGF8 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human FGF8(163-185aa FMKRLPRGHHTTEQSLRFEFLNY), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-10
Question
Can one vial of this antibody stain 50-60 slides?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
Yes. The recommended antibody concentration for IHC-P is 0.5-1 µg/mL. Therefore, it is enough to stain 50-60 slides.
Boster Scientific Support
Answered: 2019-04-17
Question
We are currently using anti-FGF8 antibody PA1216 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-06-11
Answer
The anti-FGF8 antibody (PA1216) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-06-11
