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Product Info Summary
| SKU: | PB9198 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband®
SKU/Catalog Number
PB9198
PB0194 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband® catalog # PB9198. Tested in WB applications. This antibody reacts with Human;Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9198)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human G6PD recombinant protein (Position: E315-L515). Human G6PD shares 95% and 96% amino acid (aa) sequences identity with mouse and rat G6PD, respectively.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9198 is reactive to G6PD in Human, Mouse
Observed Molecular Weight
59 kDa
Calculated molecular weight
59.3 kDa
Background of G6PD
Glucose-6-phosphate dehydrogenase, also known as G6PD or G6PDH, is an enzyme that in humans is encoded by the G6PD gene. It is mapped to Xq28. G6PD plays a key role in the production of ribose 5-phosphate and the generation of NADPH in the hexose monophosphate pathway. Because this pathway is the only NADPH-generation process in mature red cells, which lack the citric adid cycle, a genetic deficiency of G6PD is often associated with adverse physiologic effects. It has been found that aldosterone decreased G6PD expression and activity, resulting in increased oxidant stress and decreased nitric oxide levels, similar to what is observed in G6PD-deficient endothelial cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9198 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: human A549 whole cell, human MCF-7 whole cell, human Hela whole cell, human U20S whole cell, human U251 whole cell, human A431 whole cell, human HepG2 whole cell, human RT4 whole cell, mouse RAW2647 whole cell
Validation Images & Assay Conditions
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Western blot analysis of G6PD using anti-G6PD antibody (PB9198).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: human U251 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human HepG2 whole cell lysates,
Lane 8: human RT4 whole cell lysates,
Lane 9: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-G6PD antigen affinity purified polyclonal antibody (Catalog # PB9198) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for G6PD at approximately 59 kDa. The expected band size for G6PD is at 59 kDa.
Specific Publications For Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband® (PB9198)
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1 Customer Q&As for Anti-Glucose 6 Phosphate Dehydrogenase/G6PD Antibody Picoband®
Question
We are currently using anti-Glucose 6 Phosphate Dehydrogenase/G6PD antibody PB9198 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
W. Parker
Verified customer
Asked: 2018-05-15
Answer
The anti-Glucose 6 Phosphate Dehydrogenase/G6PD antibody (PB9198) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-15
