Anti-Galc Antibody

Product Name

Anti-Galc Antibody

View all Galc Antibodies

SKU/Catalog Number

A01634-2

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-Galc Antibody catalog # A01634-2. Tested in WB, IHC, IF, IP, ELISA applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

Cite This Product

Anti-Galc Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01634-2)

Host

Rabbit

Contents

500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Immunogen

E.coli-derived mouse Galc recombinant protein (Position: L342-R510).

Reactive Species

A01634-2 is reactive to Galc in Human, Mouse, Rat

Calculated molecular weight

77.3 kDa

Background of Galc

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01634-2 is guaranteed for ELISA, IP, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-400
Immunofluorescence, 1:50-400
ImmunoPrecipitation, 1:250-300
ELISA, 1:100-1000

Validation Images & Assay Conditions

There are currently no images for this product. We are working on uploading them please check back shortly or contact us to expedite our upload process for this antibody.

See full target information Galc

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