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Product Info Summary
| SKU: | A03364-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Vitamin D Binding protein/Gc Antibody Picoband®
SKU/Catalog Number
A03364-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Vitamin D Binding protein/Gc Antibody Picoband® catalog # A03364-2. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Vitamin D Binding protein/Gc Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03364-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse Gc recombinant protein (Position: L17-E256).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03364-2 is reactive to Gc in Mouse, Rat
Observed Molecular Weight
53 kDa
Calculated molecular weight
53.6 kDa
Background of Gc
Vitamin D-binding protein, also/originally known as gc-globulin (group-specific component), is a protein that in humans is encoded by the GC gene.The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03364-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat liver tissue, rat liver tissue, mouse liver tissue, mouse liver tissue, human placenta tissue
Validation Images & Assay Conditions
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Western blot analysis of Gc using anti-Gc antibody (A03364-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysate,
Lane 2: rat liver tissue lysate,
Lane 3: mouse liver tissue lysate,
Lane 4: mouse liver tissue lysate,
Lane 5: human placenta tissue lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gc antigen affinity purified polyclonal antibody (Catalog # A03364-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gc at approximately 53KD. The expected band size for Gc is at 53KD.
Specific Publications For Anti-Vitamin D Binding protein/Gc Antibody Picoband® (A03364-2)
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1 Customer Q&As for Anti-Vitamin D Binding protein/Gc Antibody Picoband®
Question
We are currently using anti-Vitamin D Binding protein/Gc antibody A03364-2 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on goat tissues as well?
R. Krishna
Verified customer
Asked: 2020-01-16
Answer
The anti-Vitamin D Binding protein/Gc antibody (A03364-2) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-16
