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- Table of Contents
GH1 antibody (A00851-2) performs well in ELISA, Flow Cytometry, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of HEL cells using anti-GH1 antibody (A00851-2).
Overlay histogram showing HEL cells stained with A00851-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GH1 Antibody (A00851-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing HEL cells stained with A00851-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-GH1 Antibody (A00851-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Designing a Western blot for GH1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the GH1 Western Blot GuideBundle Save
Anti-GH1 Antibody Picoband®
GH1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, WB |
Tested Applications
GH1 antibody was positively detected by WB in:
human HEL whole cell, human 293T whole cell, rat C6 whole cell, rat PC-12 whole cell, mouse NIH/3T3 whole cell, mouse RAW2647 whole cell, mouse 3T3-L1 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GH1 antibody was positively detected by FC in:
HEL cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-GH1 Antibody Picoband®
View all GH1 Antibodies
View all GH1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human GH1 recombinant protein (Position: Q22-F217).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
24.8 kDa
Background of GH1
Growth Hormone(GH) is mapped to 17q22-q24. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. Rat GH shares 98% amino acid sequence homology with mouse. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.
Cite This Product
Anti-GH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00851-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
68%
Low homology
Mouse
67%
Low homology
Rabbit
67%
Low homology
Rat
66%
Low homology
Cow
66%
Low homology
Zebrafish
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GH1 antibody
Flow Cytometry (FC) protocol for GH1 antibody
Review
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1 Review For Anti-GH1 Antibody Picoband®
WB analysis using Anti-GH antibody (A00851-2) in mouse hippocampal tissue revealed a specific band at the expected molecular weight with negligible non-specific signals.
| SKU | A00851-2 |
|---|---|
| Application | Western Blot |
| Sample | mouse hippocampal tissue |
| Sample Processing Description | Total protein was extracted from the left hippocampus of normal mouse brain. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Electrophoresis buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | GH1 Antibody Picoband® |
| Primary Incubation | 1:4000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | 1:10000, 1h in RT |
| Detection | Substrate: ECL substrate; Image system: ChemiDoc MP |
| Results Summary | Growth hormone (GH) serves as a central integrative signal that coordinates growth, metabolism, and tissue repair in response to changes in nutritional status. It is not only the primary driver of linear growth during puberty but also plays a critical role throughout life in maintaining muscle mass, bone strength, and metabolic flexibility. In this study, hippocampal tissues from two normal mouse brains were used to evaluate the performance of the GH antibody. The results showed a band at the expected position with good specificity, indicating that the antibody performs well in WB applications. |
Yajun Qiao, Qinghai University
Verified customer
Submitted 2026-05-06
Product Promise
We promise Anti-GH1 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A00851-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 26, 2026