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- Table of Contents
SLC2A1 antibody (PB9435) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 9 Publications
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Flow Cytometry analysis of A431 cells using anti-SLC2A1 antibody (PB9435).
Overlay histogram showing A431 cells stained with PB9435 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC2A1 Antibody (PB9435, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing A431 cells stained with PB9435 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC2A1 Antibody (PB9435, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband®
SLC2A1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
SLC2A1 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue, mouse NIH3T3 whole cell, human placenta tissue, human A431 whole cell, mouse brain tissue, rat PC-12 whole cell, mouse NIH/3T3 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC2A1 antibody was positively detected by IHC in:
Mouse Brain tissue, Rat Brain tissue, Human Placenta tissue, mouse brain tissue, rat brain tissue, rat brain tissue, human mammary cancer tissue, human placenta cancer tissue, human placenta cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SLC2A1 antibody was positively detected by IHC-F in:
Human Placenta tissue
SLC2A1 antibody was positively detected by ICC/IF in:
HepG2 cell
SLC2A1 antibody was positively detected by ICC in:
A549 cell
SLC2A1 antibody was positively detected by FC in:
A431 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human, -
Immunocytochemistry/Immunofluorescence
2μg/ml
Human, -
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Human
Tested
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband®
View all SLC2A1 Antibodies
View all SLC2A1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SLC2A1 recombinant protein (Position: R92-V492). Human SLC2A1 shares 98% and 98.3% amino acid (aa) sequence identity with mouse and rat SLC2A1, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated Molecular Weight
54.1 kDa
Background of SLC2A1
GLUT1, also known as SLC2A1, is a major glucose transporter in the mammalian blood-brain barrier whose gene is mapped to 1p35-p31.3 and contains 10 exons. It is present at high levels in primate erythrocytes and brain endothelial cells. Not only can transport dehydroascorbic acid (the oxidized form of vitamin C) into the brain, GLUT1 is also likely to contribute to HTLV-associated disorders through interacting with HTLV envelope glycoproteins. Functionally, GLUT1 deficiency causes a decrease in embryonic glucose uptake and apoptosis, which may be involved in diabetic embryopathy, by contrast, an increased expression of GLUT1 in some malignant tumors may suggest a role for glucose-derivative tracers to detect in vivo thyroid cancer metastases by positron-emission tomography scanning.
Cite This Product
Anti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9435)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Rat
98%
High Homology
Mouse
98%
High Homology
Sheep
98%
High Homology
Cow
98%
High Homology
Dog
98%
High Homology
Rabbit
97%
High Homology
Zebrafish
81%
Moderate Homology
Drosophila
50%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC2A1 antibody
Immunohistochemistry (IHC) protocol for SLC2A1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for SLC2A1 antibody
Immunocytochemistry (ICC) protocol for SLC2A1 antibody
Flow Cytometry (FC) protocol for SLC2A1 antibody
Publications
Application:
Species:
Skullcapflavone II Suppresses Colorectal Cancer by Inhibiting the HIF-1α-Glycolysis Pathway
Published: 2025-10-21
Journal: Chemical Biology & Drug Design
Application: WB
Impact Factor: 3.30
Species: Mouse,Human
PMID: 41121650
Expression of SLC2A1 is upregulated in a mouse model of age-related hearing loss
Published: 2025-03-27
Journal: CELLULAR SIGNALLING
Application: IF
Impact Factor: 3.70
PMID: 40157470
Lactate facilitates classical swine fever virus replication by enhancing cholesterol biosynthesis
Published: 2022-10-13
Journal: iScience
Application: WB
Impact Factor: 6.11
Species: Pig
PMID: 36339254
Construction of the experimental rat model of gestational diabetes
Published: 2022-09-15
Journal: PLoS One
Application: WB
Impact Factor: 3.75
Species: Rat
PMID: 36107823
Anti-tumor effect of aloe-emodin on cervical cancer cells was associated with human papillomavirus E6/E7 and glucose metabolism
Published: 2019-05-14
Journal: OncoTargets and Therapy
Application: WB
Impact Factor: 3.05
Species: Human
PMID: 31190872
Microglial activation: an important process in the onset of epilepsy
Published: 2018-09-15
Journal: American Journal of Translational Research
Application: IF
Impact Factor: 3.06
Species: Rat
PMID: 30323874
Caffeic Acid Phenethyl Ester inhibit Hepatic Fibrosis by Nitric Oxide Synthase and Cystathionine Gamma-Lyase in Rats
Published: 2015-09-17
Journal: MEDICAL SCIENCE MONITOR
Application: IHC
Impact Factor: 1.43
Species: Rat
PMID: 26378818
How does hypoxia inducible factor-1α participate in enhancing the glycolysis activity in cervical cancer?
Published: 2013-02-01
Journal: Annals of Diagnostic Pathology
Application: ELISA
Impact Factor: 0.98
Species: Human
PMID: 23375385
Necrosis Degree Displayed in Computed Tomography Images Correlated With Hypoxia and Angiogenesis in Breast Cancer
Published: 2013-01-01
Journal: JOURNAL OF COMPUTER ASSISTED TOMOGRAPHY
Application: IHC
Impact Factor: 1.58
Species: Human
PMID: 23321829
Review
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1 Review For Anti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband®
Immunocytochemistry for Anti-CD31/PECAM1 Antibody
| SKU | PB9435 |
|---|---|
| Application | Immunohistochemistry (paraffin-embedded) |
| Blocking step | 5% BSA as a blocking agent for 30 min at 37°C |
| Sample | Rat Brain Tissue |
| Fixative | Fixed with 4% paraformaldehyde |
| Primary Ab Incubation | 37°C for 30 minutes |
| Primary Ab Incubation diluent | 5% BSA in TBS |
| Primary Ab Concentration | 1ug/ml |
| Secondary Antibody | SABC kit from Boster Bio, (SA1022) |
| Secondary Ab Dilution | The kit was ready to use, no dilution needed |
| Secondary Ab Incubation | 4°C overnight |
Anti-Glucose Transporter GLUT1/SLC2A1 Antibody
Verified customer
Submitted 2019-09-12
Product Promise
We promise Anti-Glucose Transporter GLUT1/SLC2A1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
PB9435
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026