Click image to see more details
Product Info Summary
| SKU: | A07827 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-Melatonin-related receptor GPR50 Antibody
SKU/Catalog Number
A07827
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Melatonin-related receptor GPR50 Antibody catalog # A07827. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Melatonin-related receptor GPR50 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07827)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human MTR1L. AA range:301-350
Reactive Species
A07827 is reactive to GPR50 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
67.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07827 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Immunofluorescence analysis of LOVO cells, using MTR1L Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using GPR50 Polyclonal Antibody diluted at 1:1000
Specific Publications For Anti-Melatonin-related receptor GPR50 Antibody (A07827)
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Customer Q&As
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7 Customer Q&As for Anti-Melatonin-related receptor GPR50 Antibody
Question
I see that the anti-GPR50 antibody A07827 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-04-30
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adenohypophysis using anti-GPR50 antibody A07827. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-29
Question
Is this A07827 anti-GPR50 antibody reactive to the isotypes of GPR50?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
The immunogen of A07827 anti-GPR50 antibody is Synthesized peptide derived from the Internal region of human GPR50. at AA rangle: 270-350. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-14
Question
Does anti-GPR50 antibody A07827 work for WB with adenohypophysis?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
According to the expression profile of adenohypophysis, GPR50 is highly expressed in adenohypophysis. So, it is likely that anti-GPR50 antibody A07827 will work for WB with adenohypophysis.
Boster Scientific Support
Answered: 2019-07-25
Question
I was wanting to use your anti-GPR50 antibody for WB for human adenohypophysis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human adenohypophysis identification?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
It shows on the product datasheet, A07827 anti-GPR50 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adenohypophysis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-21
Question
Would A07827 anti-GPR50 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
G. Kulkarni
Verified customer
Asked: 2018-08-01
Answer
You can see on the product datasheet, A07827 anti-GPR50 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-01
Question
Do you have a BSA free version of anti-GPR50 antibody A07827 available?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GPR50 antibody A07827 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-25
