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Product Info Summary
| SKU: | A13015 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-G-protein coupled receptor 52 GPR52 Antibody
SKU/Catalog Number
A13015
Size
100μl
Form
Liquid
Description
Boster Bio Anti-G-protein coupled receptor 52 GPR52 Antibody catalog # A13015. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G-protein coupled receptor 52 GPR52 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A13015)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GPR52. AA range:311-360
Reactive Species
A13015 is reactive to GPR52 in Human, Mouse
Calculated molecular weight
41.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A13015 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis of various cells using GPR52 Polyclonal Antibody diluted at 1:2000
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Western blot analysis of 293T 3T3 lysis using GPR52 antibody. Antibody was diluted at 1:2000
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Western blot analysis of lysates from COLO, HUVEC, HeLa, and HepG2 cells, using GPR52 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-G-protein coupled receptor 52 GPR52 Antibody (A13015)
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Customer Q&As
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4 Customer Q&As for Anti-G-protein coupled receptor 52 GPR52 Antibody
Question
I was wanting to use your anti-GPR52 antibody for WB for human caudate nucleus on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human caudate nucleus identification?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
It shows on the product datasheet, A13015 anti-GPR52 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human caudate nucleus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-GPR52 antibody A13015 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
J. Lewis
Verified customer
Asked: 2018-01-04
Answer
The anti-GPR52 antibody (A13015) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-04
Question
My question regarding product A13015, anti-GPR52 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Jones
Verified customer
Asked: 2016-09-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A13015 anti-GPR52 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-09-14
Question
We want to test anti-GPR52 antibody A13015 on human caudate nucleus for research purposes, then I may be interested in using anti-GPR52 antibody A13015 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Z. Zhang
Verified customer
Asked: 2015-09-14
Answer
The products we sell, including anti-GPR52 antibody A13015, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-09-14
