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Product Info Summary
| SKU: | M06548 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody [Clone ID: OTI8H10]
SKU/Catalog Number
M06548
Size
100 µl
Description
Boster Bio GAS7 (Growth Arrest Specific Protein 7) mouse monoclonal antibody, clone OTI8H10 (formerly 8H10). Catalog# M06548. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody [Clone ID: OTI8H10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06548)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI8H10
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human GAS7 (NP_003635) produced in HEK293T cell.
Reactive Species
M06548 is reactive to GAS7 in Human, Mouse, Rat
Calculated molecular weight
54.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06548 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2000
IF: 1:100
Flow Cytometry: 1:100
IHC: 1:50-1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GAS7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-GAS7 mouse monoclonal antibody at 1:150 (M06548)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GAS7 mouse monoclonal antibody at 1:150 (M06548)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GAS7 mouse monoclonal antibody at 1:150 (M06548)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-GAS7 mouse monoclonal antibody at 1:150 (M06548)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GAS7 mouse monoclonal antibody at 1:150 (M06548)
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-GAS7 mouse monoclonal antibody at 1:150 (M06548)
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HEK293T cells transfected with either GAS7 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GAS7 antibody (M06548)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-GAS7 mouse monoclonal antibody (M06548) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GAS7.
Specific Publications For Anti-Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody [Clone ID: OTI8H10] (M06548)
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